نوع مقاله : مقاله پژوهشی
نویسنده
گروه میکروبیولوژی، واحد مراغه، دانشگاه آزاد اسلامی، مراغه، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسنده [English]
Pseudomonas aeruginosa is an important opportunistic pathogen that causes mastitis resistant to treatment in lactating dairy cows. This bacterium is a nosocomial pathogen and can be also transmitted to humans through food. Antibiotic resistance and biofilm production are two important factors in the pathogenesis and long-term persistence of Pseudomonas aeruginosa infections. Pseudomonas aeruginosa has two complete Quorum sensing systems that cause the activation of pathogenic genes. The aim of this research was to study of frequency of Quorum sensing genes in Pseudomonas aeruginosa isolated from bovine mastitis in Maragheh city in 2022. In this descriptive cross-sectional study, 200 cows with clinical mastitis were sampled and 60 Pseudomonas aeruginosa isolates were identified after biochemical and staining tests. Then the isolated samples were investigated for the presence of lasI and rhlR genes by PCR method. The frequency of lasI and rhlR genes was observed in 2 (3.33%) and 8 (13.33%) isolates, respectively. Also, 2 isolates (3.33%) harbored both lasI and rhlR genes simultaneously. In this research, the frequency of Quorum sensing genes was less than in previous studies on samples isolated from human cases, which geographical differences and ecological origin of bacterial isolates can play a significant role in justifying its cause
کلیدواژهها [English]
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