نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه دامپزشکی، واحد شوشتر، دانشگاه آزاد اسلامی، شوشتر، ایران
2 گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
To identify virulence and antibiotic resistance genes in Escherichia coli (E. coli) strains responsible for yolk sac infection in broiler chickens in Isfahan (Center of Iran), 100 culture samples were collected from the of yolk sacs from 20 farms suspected of yolk sac infection with associated mortality. The samples were cultured on MacConkey agar, and E. coli strains were confirmed using EMB agar and biochemical tests. The isolates were then cultured on Mueller Hinton agar and subjected to antibiogram testing using commercial antibiotic discs. Specific primers were used to detect the virulence genes fimC, hlyF, iss, and iutA. The results revealed that out of the 100 cultured samples, 60 E. coli strains were confirmed through culture and biochemical tests. The highest percentage of antibiotic resistance among the isolated strains was observed against tetracycline. The most frequently identified virulence gene in E. coli strains isolated from yolk sac infections was fimC (70%), while iss had the lowest frequency (35%). The most common genotype observed was fimC-hlyF-iutA-iss (71.35%), which exhibited the highest percentage of antibiotic resistance against 13 commercial antibiotics studied (92%). These findings suggest that genotypes involved in yolk sac infection exhibit significant diversity, and strains carrying virulence genes are more likely to possess higher levels of antibiotic resistance.
کلیدواژهها [English]
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