شناسایی ژن‌های حدت و مقاومت آنتی بیوتیکی در سویه‌های اشریشیاکلی جدا شده از عفونت کیسه زرده جوجه‌های گوشتی در اصفهان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه دامپزشکی، واحد شوشتر، دانشگاه آزاد اسلامی، شوشتر، ایران

2 گروه دامپزشکی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران.

3 گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران

چکیده

به منظور شناسایی ژن های حدت و مقاومت آنتی بیوتیکی در سویه های اشریشیاکلی جدا شده از عفونت کیسه زرده در جوجه های گوشتی اصفهان، 100 سویه اشریشیاکلی از 20 فارم مشکوک به عفونت کیسه زرده در سال 1402 تهیه شد. سویه ها با کشت بر روی محیط مک کانکی آگار و ائوزین متیلن بلو و نیز انجام تست های بیوشیمیایی تایید شدند. در نهایت، بر روی محیط مولر هینتون آگار کشت داده شد و در نهایت با روش دیسک دیفیوژن (کربی بائر) میزان حساسیت آنتی بیوتیکی بررسی شد. سپس با پرایمر های اختصاصی به شناسایی ژن های حدت  fimC، hlyF، iss و iutA پرداخته شد. مطابق با نتایج از 100 نمونه کشت تهیه شده 60 سویه اشریشیاکلی در کشت و تست های بیوشیمیایی تایید شد. بیشترین درصد مقاومت آنتی بیوتیکی در سویه های جدا شده علیه تتراسایکلین دیده شد. بیشترین فراوانی ژن حدت شناسایی شده در سویه های اشریشیاکلی جدا شده از عفونت کیسه زرده مربوط به fimC  بود (70 درصد) در حالیکه iss واجد کمترین درصد فراوانی بود (35 درصد). بیشترین ژنوتیپ مشاهده شده fimC-hlyF-iutA-iss  بود؛ (71/35 درصد) که بالاترین درصد مقاومت آنتی بیوتیکی نسبت به 13 آنتی بیوتیک تجاری مورد مطالعه را نشان داد (92 درصد). لذا به نظر می رسد ژنوتیپ های دخیل در عفونت کیسه زرده واجد تنوع بالایی هستند و سویه های واجد ژن های حدت بیشتر می توانند از مقاومت آنتی بیوتیکی بالاتری برخوردار باشند.
 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The identification of virulence and antibiotic resistance genes in Escherichia coli strains; isolated from yolk sac infections of broiler chickens in Isfahan

نویسندگان [English]

  • Habib Heidari 1
  • seyedeh ommolbanin ghasemian 2
  • Majid Gholami-Ahangaran 3
1 Department of Veterinary, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran
2 Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran.
3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
چکیده [English]

To identify virulence and antibiotic resistance genes in Escherichia coli (E. coli) strains responsible for yolk sac infection in broiler chickens in Isfahan (Center of Iran), 100 culture samples were collected from the of yolk sacs from 20 farms suspected of yolk sac infection. The samples were cultured on MacConkey and EMB agar, and confirmed with biochemical tests. The isolates were then cultured on Mueller Hinton agar and subjected to antibiogram testing using commercial antibiotic discs. Specific primers were used to detect the virulence genes fimC, hlyF, iss, and iutA. The results revealed that out of the 100 cultured samples, 60 E. coli strains were confirmed through culture and biochemical tests. The highest percentage of antibiotic resistance among the isolated strains was observed against tetracycline. The most frequently identified virulence gene in E. coli strains isolated from yolk sac infections was fimC (70%), while iss had the lowest frequency (35%). The most common genotype observed was fimC-hlyF-iutA-iss (71.35%), which exhibited the highest percentage of antibiotic resistance against 13 commercial antibiotics studied (92%). These findings suggest that genotypes involved in yolk sac infection exhibit significant diversity, and strains carrying virulence genes are more likely to possess higher levels of antibiotic resistance.

کلیدواژه‌ها [English]

  • Escherichia coli
  • Broiler Chickens
  • Virulence Genes
  • Antibiotic Resistance
  • Yolk Sac Infection

مراجع

Abd El Tawab, A. A., El-Hofy, F. I., Ammar, A. M., & Abdel Galil, N. M. (2016). Molecular screening of virulence genes in avian pathogenic esherichia coli. Benha Veterinary Medical Journal, 30(1), 137-149.
Delicato, E.R., Guimaraes, D.B.B., Gaziri, L.C. J., & Vidotto, M. C. (2003). Virulence-associatedgenes in Escherichia coli isolates from poultry with colibacillosis. Veterinary Microbiology, 94(2), 97-103.
Dziva, F., & Stevens, M. P. (2008). Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts. Avian Pathology, 37(4), 355-366.
Gholami-Ahangaran, M., Karimi-Dehkordi, M., Miranzadeh-Mahabadi, E., &Ahmadi-Dastgerdi, A. (2021). The frequency of tetracycline resistance genes in Escherichia coli strains isolated from healthy and diarrheic pet birds. Iranian Journal of Veterinary Research, doi: 10.22099/ijvr.2021.38454.5592
Gholami-Ahangaran, M., & Zia-Jahromi, N. (2014). Identification of shiga toxin and intimin genes in Escherichia coli detected from canary (Serinus canaria domestica). Toxicology and Industrial Health, 30(8), 724-727.
Gregova, G., Kmetova, M., Kmet, V., Venglovsky, J., & Feher, A. (2012). Antibiotic resistance of Escherichia coliisolated from a poultry slaughterhouse. Annals of Agricultural and Environmental Medicine, 19, 75-7.
Guabiraba, R., & Schouler, C. (2015). Avian colibacillosis: still many black holes. FEMS Microbiology Letters, 362(15), fnv118.
Horn, R.V., Cardoso, W.M., Lopes, E.S., Teixeira, R.S., Albuquerque, Á.H., Rocha-e-Silva, R.C., & Bezerra, W.G., (2015). Identification and antimicrobial resistance of members from the Enterobacteriaceae family isolated from canaries (Serinus canaria). Pesquisa Veterinária Brasileira, 35, 552-556.
Horri, M., & Gholami-Ahangaran, M. (2022). Phenotypic and Genotypic Characterization of Antibiotic Resistance in Escherichia coli Strains Isolated from Broiler Chickens with Suspected Colibacillosis in Isfahan Province, Iran. Infection Epidemiology and Microbiology, 8(3), 193-201.
Hussein, K. A., & Hussein, A. N. (2022). Phenotypic and genotypic characterization of the hemolysin enzyme produced by Escherichia coli isolated from different sources. In AIP Conference Proceedings (Vol. 2398, No. 1). AIP Publishing.
Janben, T., Schwarz, C., Preikschat, P., Voss, M., Philipp, H. C., & Wieler, L. H. (2001). Virulence-associated genes in avian pathogenic Escherichia coli (APEC) isolated from internal organs of poultry having died from colibacillosis. International Journal of Medical Microbiology,291, 371-378.
Jeong, K. C., Hiki, O., Kang, M. Y., Park, D., & Kaspar, C. W. (2013). Prevalent and persistent Escherichia coli O157: H7 strains on farms are selected by bovine passage. Veterinary Microbiology, 162(2-4), 912-920.
Johar, A., Al-Thani, N., Al-Hadidi, S. H., Dlissi, E., Mahmoud, M. H., & Eltai, N. O. (2021). Antibiotic resistance and virulence gene patterns associated with avian pathogenic Escherichia coli (APEC) from broiler chickens in Qatar. Antibiotics, 10(5), 564.
Johnson, J. R., & Stell, A. L. (2000). Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. The Journal of Infectious Diseases, 181(1), 261-272.
Johnson, T. J., Wannemuehler, Y., Doetkott, C., Johnson, S. J., Rosenberger, S. C., & Nolan, L. K. (2008). Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool. Journal of Clinical Microbiology, 46(12), 3987-3996.
Kathayat, D., Lokesh, D., Ranjit, S., & Rajashekara, G. (2021). Avian pathogenic Escherichia coli (APEC): an overview of virulence and pathogenesis factors, zoonotic potential, and control strategies. Pathogens, 10(4), 467.
Kim, Y. B., Yoon, M. Y., Ha, J. S., Seo, K. W., Noh, E. B., Son, S. H., & Lee, Y. J. (2020). Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis. Poultry Science, 99(2), 1088-1095.
Li, Y., Chen, L., Wu, X., & Huo, S. (2015). Molecular characterization of multidrug-resistant avian pathogenicEscherichia coli isolated from septicemic broilers. Poultry science, 94(4), 601-611.
Mahmud, Z., Shabnam, S. A., Mishu, I. D., Sadique, A., Islam, L. N., & Alam, M. (2024). Unveiling the spectrum of antibiotic resistance and virulence genes in Escherichia coli: A comparative study of clinical and environmental isolates. Gene Reports, 101987.
Murase, K., Martin, P., Porcheron, G., Houle, S., Helloin, E., Pénary, M., & Oswald, E. (2016). HlyF produced by extraintestinal pathogenic Escherichia coli is a virulence factor that regulates outer membrane vesicle biogenesis. The Journal of infectious diseases, 213(5), 856-865.
Nikpiran, H., Peighambari, M., &Abdi, A. (2018). The frequency of tsh, iutA and iss in Escherichia coli strains suspected to colibacillosis, Iranian Journal of Vetetrinary Medicine, 14 (4), 106-114 (In Persian).
Nolan, L.K. Vaillancourt, J. Barbieri, N.L. & Logue, C.M. (2020). Colibacillosis. In: Swayne, DE; Boulianne, M; Logue, CM; McDougald, LR; Nair, V and Suarez, DL (Eds.), Disease of Poultry. (14th Edn.), Massachusetts, W.B. Publishing. PP. 770-790.
Radwan, I.A., Hassan, H.S., Abd-Alwanis, S.A., &Yahia, M.A. (2014). Frequency of some virulence associated genes among multidrug-resistant Escherichia coli isolated from septicemic broiler chicken. International Journal of Advanced Research, 2(12), 867-874.
Rocha, A. C., Rocha, S. L., Lima-Rosa, C. A., Souza, G. F., Moraes, H. L., Salle, F. O., ... & Salle, C. T. (2008). Genes associated with pathogenicity of avian Escherichia coli (APEC) isolated from respiratory cases of poultry. Pesquisa Veterinária Brasileira, 28, 183-186.
Shahiri, M.R., Gholami_ahangaran, M., &Rahimi, E. (2018). The comparison of antibiotic resistance pattern in Escherichia coli strains, isolated from chickens reared on conventional and without antibiotic consumption. Iranian Journal of Food Microbiology, 5 (2), 11-18 (In Persian).
Sigirci, B. D., Celik, B., Halac, B., Adiguzel, M. C., Kekec, I., Metiner, K., ... & Kahraman, B. B. (2020). Antimicrobial resistance profiles of Escherichia coli isolated from companion birds. Journal of King Saud University-Science, 32(1), 1069-1073.
Suárez-Pérez, A., Corbera, J. A., González-Martín, M., & Tejedor-Junco, M. T. (2021). Multidrug-Resistant Phenotypes of Escherichia coli Isolates in Wild Canarian Egyptian Vultures (Neophron percnopterus majorensis). Animals, 11(06), 1692.
Trampel Van, T.T.H., Chin, J., Chapman, T., Tran, L. T., & Coloe, P.J.  (2008).  Safety of raw meat and shellfish in Vietnam: An analysis of Escherichia coliisolations for antibiotic resistance and virulence genes. International Journal of Food Microbiology, 124: 217-223.
Varga, C., Brash, M. L., Slavic, D., Boerlin, P., Ouckama, R., Weis, A., & Guerin, M. T. (2018). Evaluating virulence-associated genes and antimicrobial resistance of avian pathogenic Escherichia coli isolates from broiler and broiler breeder chickens in Ontario, Canada. Avian Diseases, 62(3), 291-299.
Won, G. Y., Moon, B. M., Oh, I. G., Matsuda, K., Chaudhari, A. A., Hur, J., ... & Lee, J. H. (2009). Profiles of virulence-associated genes of avian pathogenic Escherichia coli isolates from chickens with colibacillosis. The journal of poultry science, 46(3), 260-266.
تحقیقات آزمایشگاهی دامپزشکی
دوره 17، شماره 1 - شماره پیاپی 27
در حال تکمیل
فروردین 1404
صفحه 105-116

فایل ها

هم رسانی

ارجاع به این مقاله

آمار