نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانش آموخته دانشکده دامپزشکی، دانشگاه سمنان ،سمنان، ایران
2 استادیار گروه آموزشی درمانگاهی دانشگاه سمنان، ایران
3 Department of Basic sciences, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran
4 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه سمنان، سمنان، ایران
5 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه سمنان، سمنان، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
The aim of this study is to evaluate the antioxidant effect of melatonin on reducing the oxidative damage of alcohol in the testes of adult rats. In this study, the animals under investigation were divided into four groups : Group 1 (negative control): Daily administration of normal saline orally (3 gr/kg) and intraperitoneal injection of normal saline for 28 days; Group 2 (positive control): Induction of testicular oxidative damage using a daily oral dose of 30% ethanol solution (3 gr/kg) and intraperitoneal injection of normal saline for 28 days; Group 3 (intervention with subclinical dose of melatonin): Daily oral administration of ethanol (3 gr/kg) along with intraperitoneal injection of melatonin at a dose of 5 mg/kg to prevent oxidative damage for 28 days; Group 4 (intervention with clinical dose of melatonin): Daily oral administration of ethanol (3 gr/kg) along with intraperitoneal injection of melatonin at a dose of 10 mg/kg for 28 days. 1% ethanol was used for melatonin solubility. At the end of the study, blood samples were taken from rats and testosterone concentration was measured by CLIA method. The testes were removed and after weighing, they were fixed in 10% formalin buffer solution for 72 hours. After processing with standard histological methods and paraffin molding, sections with a thickness of 5 microns were prepared and after H&E staining, the his tomorphometric study was performed. Ethanol significantly reduced testicular weight, serum testosterone concentration, and histomorphometric parameters compared to the negative control group. Combined treatment of melatonin with subclinical dose and ethanol resulted in a significant increase in testicular weight, serum testosterone concentration, and mean number of primary spermatocytes and spermatids compared to the positive control group, but other histomorphometric parameters did not change significantly. Combined treatment of melatonin with clinical dose and ethanol resulted in a significant increase in testicular weight, serum testosterone concentration, and all histomorphometric parameters in comparison with the positive control group. It seems that intraperitoneal administration of melatonin at a clinical dose for 28 days is an important factor in improving oxidative damage to testicular tissue by reducing oxidative stress.
کلیدواژه‌ها [English]
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