نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانش آموخته دانشکده دامپزشکی، دانشگاه سمنان ،سمنان، ایران
2 استادیار گروه آموزشی درمانگاهی دانشگاه سمنان، ایران
3 Department of Basic sciences, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran
4 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه سمنان، سمنان، ایران
5 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه سمنان، سمنان، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
This study aimed to assess the protective role of melatonin against ethanol-induced oxidative damage in the testes of adult rats. Thirty-two rats were randomly assigned into four groups: Group 1 (negative control) received normal saline orally and intraperitoneally; Group 2 (positive control) received 30% ethanol (3 g/kg orally) and saline intraperitoneally; Group 3 received ethanol plus melatonin at a subclinical dose (5 mg/kg IP); Group 4 received ethanol plus melatonin at a clinical dose (10 mg/kg IP). Blood samples were collected for serum testosterone analysis by CLIA, and testes were harvested, weighed, and histologically processed. Ethanol significantly decreased testicular weight, serum testosterone levels, and histomorphometric indices compared to controls. The group receiving 5 mg/kg melatonin showed significant improvements in testicular weight, testosterone levels, and the number of primary spermatocytes and spermatids, though other parameters remained unchanged. In contrast, the 10 mg/kg melatonin group exhibited significant improvements in all measured parameters. These findings suggest that melatonin, particularly at clinical doses, may effectively mitigate ethanol-induced testicular damage by reducing oxidative stress and enhancing structural and hormonal recovery of the reproductive system.
کلیدواژه‌ها [English]
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