بررسی شیوع سارکوسیست با روش‌های میکروسکوپی، ماکروسکوپی و واکنش زنجیره ای پلیمراز (PCR) در دام‌های کشتارشده در کشتارگاه اصفهان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه ازاد شهرکرد

2 استاد تمام، بهداشت مواد غذایی

چکیده

انگل‌های جنس سارکوسیست از رایج‌ترین انگل‌های موجود در نشخوارکنندگان اهلی هستند و برخی از آن­ها می‌توانند در هنگام ایجاد بیماری‌های بالینی و تحت بالینی زیان اقتصادی مهمی به وجود بیاورند. هدف از پژوهش حاضر بررسی میزان آلودگی سارکوسیست با استفاده از روش­های میکروسکوپی، ماکروسکوپی و PCR در دام­های کشتارشده در کشتارگاه اصفهان بود. در این پژوهش 335  لاشه شامل 130 لاشه گوسفند، 95 لاشه بز و 110 لاشه گاو جهت بررسی آلودگی مورد ارزیابی قرار گرفتند. بافت­های ارزیابی­شده شامل زبان، مری، قلب و دیافراگم بود. نتایج نشان داد از 335 نمونه لاشه دام کشتارشده، در روش ماکروسکوپی 18 نمونه (37/5 درصد)، در روش میکروسکوپی 46 نمونه (73/13 درصد) و در روش PCR 74 نمونه (08/22 درصد) سارکوسیست تشخیص داده شد. نتایج نشان داد میزان آلودگی در لاشه گوسفند 62 نمونه (62/47 درصد)، لاشه گاو 28 نمونه (18/28درصد) و لاشه بز 45 نمونه (36/47 درصد) به سارکوسیست آلوده بودند. بیشترین فراوانی سارکوسیست به ترتیب در نمونه گوسفند در مری (22/53 درصد) و کمترین در قلب و دیافراگم (67/9 درصد)، برای گاو بیشترین فراوانی سارکوسیست در زبان (14/48 درصد) و کمترین در دیافراگم (87/9 درصد) و برای بز بیشترین فراوانی سارکوسیست در مری (11/31 درصد) و کمترین فراوانی در دیافراگم (56/15 درصد) بود. با توجه به فراوانی آلودگی به سارکوسیست در لاشه دام­های کشتارشده، توصیه به عدم مصرف گوشت و فرآورده­های گوشتی به صورت خام و نیم­پز می­شود و همچنین از نگهداری ناقلین کیست­ها در محیط نگهداری دام­ها جلوگیری شود.
 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigating the prevalence of sarcocysts by microscopic, macroscopic and PCR methods in slaughtered animals in Isfahan slaughterhouse

نویسندگان [English]

  • Fateme Abedi 1
  • Ebrahim Rahimi 2
1 Shahrekord azad university
2 professor food hygiene and quality control
چکیده [English]

Parasites of the genus Sarcocyst are among the most common parasites found in domestic ruminants, and some of them can cause significant economic losses when causing clinical and subclinical diseases. The purpose of this study was to investigate the level of sarcocyst contamination using microscopic, macroscopic and PCR methods in slaughtered animals in Isfahan slaughterhouse. In this research, 335 carcasses including 130 sheep carcasses, 95 goat carcasses and 110 cow carcasses were evaluated for contamination. The evaluated tissues included tongue, esophagus, heart and diaphragm. The results showed that out of 335 samples of slaughtered animal carcasses, 18 samples (5.37%) were diagnosed as sarcocysts in the macroscopic method, 46 samples (13.73%) in the microscopic method, and 74 samples (22.08%) in the PCR method. The results showed that 62 sheep carcasses (47.62%), 28 cattle carcasses (28.18%), and 45 goat carcasses (47.36%) were infected with sarcocyst. The highest frequency of sarcocyst in the sheep sample is in the esophagus (53.22%) and the lowest in the heart and diaphragm (9.67%), respectively, for the cow, the highest frequency of sarcocyst is in the tongue (48.14%) and the lowest in the diaphragm (9.87%) and for goats, the highest frequency of sarcocysts was in the esophagus (31.11%) and the lowest frequency was in the diaphragm (15.56%). Considering the frequency of sarcocyst infection in the carcasses of slaughtered animals, it is recommended not to consume meat and meat products in raw and semi-cooked form, and also to avoid keeping cyst carriers in the environment of keeping animals.

کلیدواژه‌ها [English]

  • Meat
  • Sarcocyst
  • Microscopy
  • Macroscopic
  • PCR

مراجع

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