نوع مقاله : مقاله پژوهشی
نویسنده
دانشکده دامپزشکی دانشگاه سمنان
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسنده [English]
Glioblastoma is a type of malignant and invasive glioma. This is the second most common CNS tumor in dogs and the third or fourth most common tumor in cats. Much of the current knowledge about gliomas in animals is derived from the fact that these tumors share significant clinical and pathological features with their human counterparts. Among these common features, we can mention necrosis and vascular proliferation in this tumor. Its clinical symptoms can include ataxia, pressing the head against hard objects, turning the head to one side and nystagmus.
A female DSH cat, 8 years old, weighing about 4 kg, presented with neurological symptoms, head pressure against the wall, and nystagmus. She died after three days and was referred for a necropsy. The brain necropsy revealed a mass in the temporal lobe. In order to prepare histopathological sections and immunochemical tests, appropriate tissue samples were separated from the tumor mass and fixed in 10% buffered formalin. After stabilization, the samples were placed in paraffin molds by going through different and usual stages of tissue processing. Tissue sections with a thickness of 4 microns were prepared and stained by routine E&H method. Also, for immunohistochemistry, GFAP and S100 staining were done after preparation of tissue section by routine method. Glioblastoma was diagnosed after taking samples, observing H&E histopathology sections and positive immunohistochemical tests.
کلیدواژهها [English]
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