بررسی اثر آبسزیک اسید بر تغییرات هیستوپاتولوژیک متعاقب آسیب نخاعی حاد در مدل موش صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کرمان دانشگاه شهید باهنر

2 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید باهنر کرمان، کرمان، ایران

چکیده

بلافاصله پس از بروز ضایعه نخاعی، التهاب عصبی سبب درد نوروپاتیک و پیشرفت نقص حرکتی می شود.  آبسزیک اسید به عنوان یک فیتوهورمون با نقش تعدیل کنندگی در گیاهان، دارای اثرات ضد التهابی در بافت های مختلف حیوانی است.  در این پژوهش اثرات آبسزیک اسید به عنوان یک ماده ضد التهابی در مدل حاد ضایعه نخاعی مورد استفاده قرار گرفت.
برای ایجاد مدل ضایعه نخاعی در موش صحرایی از روش پرتاب وزنه استفاده شد. برای این منظور تعداد 36 موش صحرایی بالغ نر استفاده شدگروهبندی حیوانات  بصورت زیر بود گروه کنترل: فاقد آسیب و تجویز دارویی، گروه آسیب نخاعی فاقد تیمار دارویی، گروه آسیب نخاعی با تجویز بافرحلال داخل نخاعی، گروه آسیب نخاعی با تیمار آبسزیک اسید (μg/rat.IT 10(،  گروه آسیب نخاعی با تیمار μg/rat.IT) 15)، گروه آسیب نخاعی با تیمار متیل پردنیزلونmg/kg. IP) 30). برای ارزیابی اثرات آبسزیک اسید بر تغییرات پاتولوژیک در فاز حاد ضایعه نخاعی, از بافت نخاع در محل ضایعه برش گیری شد و توسط پاتولوژیست مورد بررسی قرار گرفت.
این مطالعه نشان داد که تجویز آبسزیک اسید سبب بهبودی در میزان ادم و خونریزی و نکروز در سطح پاتولوژیک در گروه های القا ضایعه نخاعی در مقایسه با گروه کنترل می گردد، اگرچه این تفاوت معنی دار نبود.
یافته های هیستوپاتولوژیک حاصل از مطالعه حاضر نشان داد که  آبسزیک اسید میتواند تنها تا حدودی در کاهش آثار پاتولوژیک در فاز حاد آسیب نخاعی عمل کند و مطالعات تکمیلی در این زمینه لازم است..

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigating the effect of abscisic acid on histopathological changes following acute spinal cord injury in a rat model

نویسندگان [English]

  • Maryam Rezaeezadeh Roukerd 1
  • Shahrzad Azizi 2
  • ساحل متقی 1
1 Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
2 Department of Pathobiology,, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
چکیده [English]

Immediately after spinal cord injury, nerve inflammation causes neuropathic pain and motor impairment. As a phytohormone with a modulating role in plants, abscisic acid has anti-inflammatory effects in various animal tissues. In this study, the effects of abscisic acid as an anti-inflammatory agent were used in the acute model of spinal cord injury.
The weight throwing method was used to create a model of spinal cord injury in rats. For this purpose, 36 adult male rats were used and the animals were grouped as follows: the control group: no injury and drug administration, spinal cord injury group without drug treatment, spinal cord injury group with intrathecal administration of fentanyl, spinal cord injury group with abscisic acid treatment (10μg /rat. IT), spinal cord injury group treated with 15μg/rat.IT), spinal cord injury group treated with methylprednisolone (30mg/kg. IP). To evaluate the effects of abscisic acid on pathological changes in In the acute phase of spinal cord injury, the spinal cord tissues were cut at the lesion site and examined by a pathologist. This study showed that the administration of abscisic acid causes improvement in the amount of edema, bleeding, and necrosis at the pathological level in the spinal cord injury induction groups compared to the control group, although this difference was not significant.
The histopathological findings from the present study showed that abscisic acid can reduce the pathological effects in the acute phase of spinal cord injury to some extend and further studies are necessary in this field.

کلیدواژه‌ها [English]

  • Abscisic acid
  • pathological changes
  • spinal cord injury

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