Molecular investigation of qnr A-B genes isolated from antibiotic-resistant Escherichia coli causing mastitis in dairy cows

Document Type : Original Article

Authors

1 1- Assistant Professor, Department of Microbiology, Department of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University, Kazerun Branch, Kazerun, Iran

2 Student of Veterinary Medicine.Department of Veterinary Medicine. Faculty of Veterinary Medicine.Islamic Azad University.Kazerun Branch.Kazerun.Iran

3 Instructor of Veterinary Physiology Medicine.Department of Veterinary Medicine. Faculty of Veterinary Medicine.Islamic Azad University.Kazerun

4 student of doctorate in veterinary medicine, clinic department, faculty of veterinary medicine, Kazerun branch, Islamic Azad University, Kazerun, Iran

Abstract

Mastitis is an important disease that can infect livestock throughout their life. Gram-negative EscherichiaColi is the main cause of most of this disease. The aim is to investigate EscherichiaColi isolates containing fluoroquinolone antibiotic resistance genes in mastitis milk using multiplex PCR technique. In this study,150milk samples from cows suffering from mastitis were used. The samples were sent to the microbiology laboratory of Faculty of Veterinary Medicine of Kazeroon University for evaluation. After culturing the suspected EscherichiaColi colonies, according to the CLSI tables, the sensitivity of the isolated strains was evaluated. The results of electrophoresis of PCR products showed that among the 7 strains of Escherichia coli resistant to flumoquine; The qnrS gene was observed in one strain and the qnrB gene was observed in one strain, and one strain had both qnrS and qnrB genes. In this study, among 17 isolates of Escherichia coli resistant to enrofloxacin (36.36%), 1 isolate has qnrB gene, 1 isolate has qnrS gene and 1 isolate has both qnrS and qnrB genes. occurrence of resistance to quinolones in Escherichia coli pathogenic strains can be a danger to public health. The most abundant pattern of multiple resistance obtained was related to the strains that were resistant to several antibiotics at the same time.The results showed that qnr S and qnrB genes are the most important genetic factors involved in resistance against quinolones and fluoroquinolones.

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References

Alessiani, A., Di Giannatale, E., Perilli, M., Forcella, C., Amicosante, G., & Zilli, K. (2009). Preliminary investigations into fluoroquinolone resistance in Escherichia coli strains resistant to nalidixic acid isolated from animal faeces. Veterinaria Italiana, 45(4), 521-527.
Burvenich, C., Van Merris, V., Mehrzad, J., Diez-Fraile, A., & Duchateau, L. (2003). Severity of E. coli mastitis is mainly determined by cow factors. Veterinary research, 34(5), 521-564.
Cano, M., Rodriguez-Martinez, J., Aguero, J., Pascual, A., & Martinez-Martinez, L. (2006). Detection of qnrS in clinical isolates of Enterobacter cloacae in Spain. Clin Microbiol Infect, 12(supp. 4), 12.
Cattoir, V., Poirel, L., Rotimi, V., Soussy, C.-J., & Nordmann, P. (2007). Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates. Journal of Antimicrobial Chemotherapy, 60(2), 394-397.
Dahmen, S., Poirel, L., Mansour, W., Bouallegue, O., & Nordmann, P. (2010). Prevalence of plasmid-mediated quinolone resistance determinants in Enterobacteriaceae from Tunisia. Clinical Microbiology and Infection, 16(7), 1019-1023.
Firoozeh, F., Zibaei, M., & Soleimani-Asl, Y. (2014). Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran. The Journal of Infection in Developing Countries, 8(07), 818-822.
Flach, C.-F., Boulund, F., Kristiansson, E., & Larsson, D. (2013). Functional verification of computationally predicted qnr genes. Annals of clinical microbiology and antimicrobials, 12(1), 1-4.
Forcella, C., Alessiani, A., Perilli, M., Zilli, K., Di Giannatale, E., & Amicosante, G. (2010). Characterization of quinolone resistance in Escherichia coli strains of animal origin from Italy. Journal of Chemotherapy, 22(3), 165-168.
Fattahi, H., Pikar, M., pourMohammadi, M., dabiri, E. Gorgin, Z..(1400). General Veterinary Microbiology. Norbakhsh press.
Levine, M. M. (1987). Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. Journal of infectious Diseases, 155(3), 377-389.
Li, X.-Z. (2005). Quinolone resistance in bacteria: emphasis on plasmid-mediated mechanisms. International journal of antimicrobial agents, 25(6), 453-463.
Lim, S.-K., Lim, K.-G., Lee, H.-S., Jung, S.-C., Kang, M.-I., & Nam, H.-M. (2010). Prevalence and molecular characterization of fluoroquinolone-resistant Escherichia coli isolated from diarrheic cattle in Korea. Journal of Veterinary Medical Science, 72(5), 611-614.
Mahon, C. R., & Lehman, D. C. (2022). Textbook of diagnostic microbiology-e-book: Elsevier Health Sciences.
Mirzaii, M., Jamshidi, S., Zamanzadeh, M., Marashifard, M., Hosseini, S. A. A. M., Haeili, M., . . . Khoramrooz, S. S. (2018). Determination of gyrA and parC mutations and prevalence of plasmid-mediated quinolone resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from patients with urinary tract infection in Iran. Journal of global antimicrobial resistance, 13, 197-200.
Quinn, P., Markey, B. K., Carter, M., Donnelly, W., & Leonard, F. (2002). Veterinary microbiology and microbial disease: Blackwell science.
Ramazan zadeh Rashid. Prevalence and characterization of extended-spectrum beta-lactamase production in clinical isolates of Klebsiella spp. African Journal of Microbiology Research. 4 July 2010; Vol. 4 (13): pp 1359-1362.
Robicsek, A., Jacoby, G. A., & Hooper, D. C. (2006). The worldwide emergence of plasmid-mediated quinolone resistance. The Lancet infectious diseases, 6(10), 629-640.
Rodríguez-Martínez, J. M., Cano, M. E., Velasco, C., Martínez-Martínez, L., & Pascual, A. (2011). Plasmid-mediated quinolone resistance: an update. Journal of Infection and Chemotherapy, 17, 149-182.
Saei, M., Jamshidi, A., Zeinali, T., & Khoramian, B. (2022). Phenotypic and genotypic determination of β-lactamase-producing Escherichia coli strains isolated from raw milk and clinical mastitis samples, Mashhad, Iran. International dairy journal, 133, 105406.
Vasilaki, O., Ntokou, E., Ikonomidis, A., Sofianou, D., Frantzidou, F., Alexiou-Daniel, S., . . . Pournaras, S. (2008). Emergence of the plasmid-mediated quinolone resistance gene qnrS1 in Escherichia coli isolates in Greece. Antimicrobial agents and chemotherapy, 52(8), 2996.
Veldman, K., Cavaco, L. M., Mevius, D., Battisti, A., Franco, A., Botteldoorn, N., . . . De Frutos Escobar, C. (2011). International collaborative study on the occurrence of plasmid-mediated quinolone resistance in Salmonella enterica and Escherichia coli isolated from animals, humans, food and the environment in 13 European countries. Journal of Antimicrobial Chemotherapy, 66(6), 1278-1286.
Wang, A., Yang, Y., Lu, Q., Wang, Y., Chen, Y., Deng, L., . . . Wang, C. (2008). Presence of qnr gene in Escherichia coli and Klebsiella pneumoniae resistant to ciprofloxacin isolated from pediatric patients in China. BMC Infectious Diseases, 8, 1-6.
Yousefi, S., Mojtahedi, A., & Shenagari, M. (2018). A survey of gyrA target-site mutation and qnr genes among clinical isolates of Escherichia coli in the north of Iran. Jundishapur journal of microbiology, 11(9).
Yue, L., Jiang, H.-X., Liao, X.-P., Liu, J.-H., Li, S.-J., Chen, X.-Y., . . . Liu, Y.-H. (2008). Prevalence of plasmid-mediated quinolone resistance qnr genes in poultry and swine clinical isolates of Escherichia coli. Veterinary microbiology, 132(3-4), 414-420.
Zaatout, N. (2022). An overview on mastitis-associated Escherichia coli: Pathogenicity, host immunity and the use of alternative therapies. Microbiological research, 256, 126960.
Journal of Veterinary Laboratory Research
Volume 14, Issue 2 - Serial Number 22
February 2023
Pages 139-150

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