تحقیقات آزمایشگاهی دامپزشکی

تحقیقات آزمایشگاهی دامپزشکی

بررسی اثرات دارو‌های دیازپام و سرترالین بر بیان ژن در افسردگی ناشی از استرس مزمن در ماهی زبرا (Danio rerio) به‌عنوان مدل حیوانی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه دامپزشکی، واحد بابل، دانشگاه آزاد اسلامی، بابل، ایران
2 گروه زیست شناسی، واحد بابل، دانشگاه آزاد اسلامی، بابل، ایران
10.22075/jvlr.2026.37436.1162
چکیده
یکی از بیماری‌های ذهنی که امروزه بسیاری از مردم جهان را تحت تأثیر قرار داده است، افسردگی می‌باشد. طبق آمار‌ها، این مشکل هر روز جدی‌تر می‌شود که نیاز روز‌افزون به تحقیق بر روی مدل‌های افسردگی را نمایان می‌کند. هدف از این تحقیق، بررسی تأثیرات دیازپام و سرترالین بر پارامتر‌های بیوشیمیایی و مولکولی در ماهی زبرا (Danio rerio) با استفاده از پروتکل UCS (Unpredictable Chronic Stress) می‏باشد. آزمایش‌های بیوشیمیایی با اندازه‌گیری کورتیزول و آزمایشات مولکولی با بررسی بیان ژن سایتوکاین‌های پیش‌التهابی و ضد‌التهابی انجام شد. نتایج این پژوهش نشان می‌دهد که دیازپام، سرترالین و ترکیب آن­ها به شکل معنی­داری موجب پیشگیری از اثرات افسردگی با کاهش معنی‌دار در میزان بیان ژن سایتوکاین­های پیش­التهابی (TNF-α, IL-6) می‌گردد. همچنین سرترالین بیشترین اثر مهاری را بر میزان کورتیزول داشته و موجب کاهش معنی­دار سطوح آن (از حدود ۱ نانوگرم کورتیزول بر ‌گرم بافت به ۰.۲۵ نانوگرم بر ‌گرم بافت) می­‌گردد. در این پژوهش داده‌های قبلی تأیید شد که نشان می‌دهد پروتکل UCS موجب القای تغییرات رفتاری و نوروآندوکرین در ماهی زبرا می‌گردد که با مصرف دیازپام و سرترالین، می‌توان از آن‌ها پیشگیری نمود. همچنین این مطالعه استفاده از ماهی زبرا به‌عنوان ارگانیسم مدل در مطالعات تأثیرات رفتاری و فیزیولوژیک استرس را تقویت نمود.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Investigation of the Effects of Diazepam and Sertraline on Gene Expression in Chronic Stress-Induced Depression in Zebrafish (Danio rerio) as an Animal Model

نویسندگان English

Shahab Tirgar fakheri 1
Reza Changizi 1
Hamed Manouchehri 1
Ali Tahamtan nezhad emran 1
Mohadeseh Najarzadeh Ahangarkolaei 1
Meysam Rameshgar 2
1 Department of Veterinary, Babol branch, Islamic Azad University, Babol, Iran
2 Department of Biology, Babol branch, Islamic Azad University, Babol, Iran
چکیده English

Depression is a prevalent mental disorder affecting millions of people worldwide, and its incidence continues to rise, highlighting the need for research using reliable depression models. This study aimed to investigate the effects of diazepam and sertraline on biochemical and molecular parameters in zebrafish (Danio rerio) subjected to the Unpredictable Chronic Stress (UCS) protocol. Biochemical analyses were conducted by measuring cortisol levels, and molecular analyses were performed by assessing the expression of pro- and anti-inflammatory cytokine genes. The results demonstrated that diazepam, sertraline, and their combination significantly prevented depression-related effects by reducing the expression of pro-inflammatory cytokine genes (TNF-α, IL-6). Sertraline exhibited the strongest inhibitory effect on cortisol, significantly lowering its levels from approximately 1 ng/g tissue to 0.25 ng/g tissue. These findings confirm previous data indicating that the UCS protocol induces behavioral and neuroendocrine changes in zebrafish, which can be mitigated by diazepam and sertraline administration. Furthermore, this study reinforces the use of zebrafish as a model organism for investigating the behavioral and physiological effects of stress.

کلیدواژه‌ها English

Depression
Diazepam
Sertraline
Zebrafish
UCS Protocol
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