تحقیقات آزمایشگاهی دامپزشکی

تحقیقات آزمایشگاهی دامپزشکی

غنی‌سازی و بهینه‌سازی ویژگی‏های کیفی دوغ حاوی کنسانتره پروتئین آب‌پنیر، جلبک اسپیرولینا پلاتنسیس (Spirulina platensis) و پودر بولاغ اوتی (Nasturtium officinale)

نوع مقاله : مقاله پژوهشی

نویسندگان
گروه بهداشت، دانشکده دامپزشکی، دانشگاه سمنان، سمنان، ایران
10.22075/jvlr.2026.38862.1185
چکیده
دوغ نوشیدنی لبنی ارزشمندی است اما در طی نگهداری با چالش‌هایی مانند دوفاز شدن و ته‌نشینی، کاهش pH و افت ماندگاری روبرو است، لذا تحقیقات گسترده‌ای در راستای افزایش مدت نگهداری و کاهش تغییرات کیفی و همچنین غنی‌سازی این محصول صورت گرفته است. هدف از این پژوهش نیز، بررسی اثرات افزودن گیاه بولاغ اوتی، جلبک اسپیرولینا پلاتنسیس و کنسانتره پروتئین آب‌پنیر بر همین ویژگی‏های کیفی دوغ بود. در این راستا، 18 نمونه دوغ شامل جلبک اسپیرولینا (0 و 4/0 و 8/0 % وزنی/ وزنی)، کنسانتره پروتئین آب‌پنیر (0 و 2 و 4 % وزنی/وزنی) و پودر بولاغ اوتی (0 و 02/0 و 04/0 % وزنی/وزنی) تولید و به‏مدت 21 روز نگهداری شدند. نتایج نشان دادند کنسانتره پروتئین آب‌پنیر و جلبک اسپیرولینا موجب افزایش معنی‌دار pH و ویسکوزیته شدند (05/0 p <). بالاترین pH برابر با 4/4 و بالاترین ویسکوزیته برابر با cp 4/9 در غلظت‏های (4% کنسانتره پروتئین آب‌پنیر 8/0 % اسپیرولینا) مشاهده شدند. کنسانتره پروتئین آب‌پنیر تا 3 % موجب افزایش پایداری فازی دوغ شد و در مقادیر بیشتر، به علت افزایش ماده خشک دوغ، پایداری فازی بطور معنی‌داری کاهش یافت (05/0 p <). بر اساس بهینه‌سازی نهایی، نمونه حاوی 95/1 % کنسانتره پروتئین آب‌پنیر، 32/0% جلبک اسپیرولینا پلاتنسیس و 02/0% پودر بولاغ اوتی به‌عنوان برترین نمونه معرفی گردید.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Enrichment and optimization of quality characteristics of Doogh (persian yoghourt drink) containing whey protein concentrate, Spirulina platensis algae and Nasturtium Officinale powder

نویسندگان English

Mohammad Hossein Khalili Samani
Azadeh Salimi
Mahnoosh Parsaeimehr
Sara Mehdizadeh Mood
Department of Hygiene, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
چکیده English

Doogh is a valuable dairy beverage, but during its storage there are some challenges such as phase separation, sedimentation, pH drops and shelf-life reduction, so nowadays many investigations are focused on reducing undesirable quality changes, enrichment and increasing its shelf life. This study aimed to evaluate the effects of Nasturtium officinale, Spirulina platensis algae, and whey protein concentrate on Doogh quality. In this regard, 18 Doogh samples were produced with Spirulina platensis (0, 0.4, and 0.8% w/w), whey protein concentrate (0, 2, and 4% w/w), and Nasturtium officinale powder (0, 0.2, and 0.04% w/w) and stored for 21 days. Results showed that whey protein concentrate and Spirulina platensis significantly increased pH and viscosity (p < 0.05). The highest pH was 4.4 and the highest viscosity was 4.9 cp at the concentrations of 4% whey protein concentrate and 0.8% Spirulina platensis. Whey protein concentrate up to 3% improved the phase stability of Doogh, while at higher amounts, due to increased solid content, phase stability decreased significantly (p < 0.05). Based on the final optimization, the sample containing 1.95% whey protein concentrate, 0.32% Spirulina platensis, and 0.02% Nasturtium officinale powder was identified as the best sample.

کلیدواژه‌ها English

Doogh
shelflife
Nasturtium Officinale
Spirulina platensis
whey protein concentrate
Abd El-Salam, M. H., El-Shibiny, S. & Salem, A. (2009). Factors affecting the functional properties of whey protein products: A review. Food Reviews International, 25(3), 251-270. DOI:10.1080/87559120902956224
Abdellatif, f., Boudgella, H., Zitouni, A. & Hassani., A. (2014). Chemical Composition and Antimicrobial Activity of the Essential Oil From leaves of Algerian Melissa Officinalis. Excli journal, 13, 772-781. PMID: 26417300
Azary, Z. & Nasirpour, A. (2018). Effect of whey protein concentrate, pH and salt on colloidal stability of acid dairy drink (Doogh). International Journal of Dairy Technology, 71(1): 198-207.
Babakhani, Z., Karami, M. & Rezazad Bari, M. (2018). The Use of micro-algae Spirulina platensis in formulation of functional fortified low-calorie sauce with iron and zinc. Journal of food science and technology, 15 (84), 125-136.
 Bhanu Prakash Goud, B., Gouthami, G., Kaushiki, K. & Venkat Reddy, K. (2024). Development and nutritional analysis of spirulina (Arthrospira platensis) based smoothie mix powder. International Journal of Advanced Biochemistry Research, 8(2), 1273-1278.  DOI:10.33545/26174693.2024.v8.i12p.3405.
Bodpa, A., & Saghir, A. (2015). Effect of whey protein products on microbiological characteristics of buffalo meat emulsion sausage. Iranian Food Science and Technology Research Journal. 3(11):260-272.
Carocho, M. & Ferreira, IC. (2013). A review on antioxidants, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food and Chemical Toxicology, 51, 15-25. DOI:10.1016/j.fct.2012.09.021.
Conway, V., Gauthier, S. F. & Pouliot, Y. (2014). Buttermilk: Much more than a source of milk pHospHolipids, Animal Frontiers,4 (2), 44-51. DOI:10.2527/af.2014-0014.
Ehtiati, A., Shahidi, F., Mohebbi, M & Yavarmanesh, M. (2013). Evaluation of WPC and EPS Producing Starter Cultures Effect on some Physical Properties of Doogh. Iranian food science and technology research journal, 9(4), 295-303 (In Persian). DOI:10.22067/ifstrj.v9i4.30072
Eslami Meshkenani, A., Fadaei Noghani, V., Khosravi Darani, K. & Mazinani, S. (2015). The effect of powdered Spirulina platensis biomass on some physicochemical and sensory properties of probiotic doogh containing powdered mint. Innovative food technologies, 2(2), 59-70 (In Persian). DOI:10.22104/jift.2015.87
Gangurde, H.H., Chordiya, M.A., Patil, P. S. and Baste, N. S. (2011). Whey protein. Scholars Research Journal, 2(1), 69-77. DOI:10.4103/2249-5975.99663
Jalalvand, P., Lavasani, S. & Eyvaz zadeh, O. (2023). The Study Effect of Spirulina platensis on Physicochemical, Microbial and Sensory Properties of Probiotic Dough Containing Lepidium sativum Seed Gum. Journal of Innovation in Food Science and Technology, 14 (4), 133-146 (In Persian). DOI:10.30495/jfst.2021.684163
Khuri, A. I. & Mukhopadhyay, S. (2010). Response surface methodology. WIREs Computational Statics, 2 (2), 128-149. DOI:10.1002/wics.73.
Kiros, E., Seifu, E., Bultosa, G., & Solomon, W. K. (2016). Effect of carrot juice and stabilizer on the pHysicochemical and microbiological properties of yoghurt. LWT-Food Science and Technology, 69, 191-196.‏ DOI:10.1016/j.lwt.2016.01.026.
Koksoy,A.& Kilik,M. (2003). Effects of water and salt level on rheological properties of ayron, a turkish yogurt drink. International dairy journal, 13/ 835-839.
Manafi Dizaj Yekan, M., Manafi Dizaj Yekan, M., Hesari, J., Azadmard-Damirchi, S. & Ravash, N. (2021). Effect of chitosan on the physico- chemical, microbiological and sensory properties of doogh. Iranian Journal of Food science and technology, 112 (18), 169-178. (In Persian). DOI:10.52547/fsct.18.112.169
National Standard of Iran, 2802, first edition 1380, Milk and its products - Determination of acidity and pH - Test method.
Nikan, j , Khavari , h. (2014). In vitro anti-fungal activity of watercress (Nasturtium officinale) extract against Fusarium solani, the causal agent of potato dry rot, Journal of Medicinal Herbs, 5(1):19-24. 
Osouli Zadeh Noubari, M., Yousefi, S. & Weisani, V. (2022). essential oils on the physicochemical, microbial, rheological and sensory properties of probiotic Doogh. Journal of food science and technology (Iran), 19 (124), 113-126 (In Persian). ‎ DOI:10.52547/fsct.19.124.113
Ozen, A. E. & Kilic, M. (2009). Improvement of physical properties of nonfat fermented milk drink by using whey protein concentrate. Journal of Texture Studies, 40(3), 288-299. DOI:10.1111/j.1745-4603.2009.00182.x
Puvanenthiran, A., Williams, R. P. W. & Augustin, M. A. (2002). Structure and Visco-elastic properties of set yoghurt with altered casein to whey protein ratios. International Dairy Journal, 12(4), 383-391. DOI:10.1016/S0958-6946(02)00033-X
Rasouli, F., Berenji, SH. & Shahab Lavasani, (2017). Optimization of traditional Iranian ice cream formula containing Spirulina platensis by response surface methodology. Food technology & nutrition, 3 (14), 15-28.
Romano, I., Bellitti, M. R., Nicolaus, B., Lama, L., Manca, M. C., Pagnotta, E. & Gambacorta, A. (2000). Lipid profile a useful chemotaxonomic marker for classification of a new cyanobacterium in Spirulina genus. Phytochemistry, 54(3), 289-294. DOI:10.1016/s0031-9422(00)00090-x