Comparison of the accuracy, sensitivity and characteristic of the wet mount and PCR method in the diagnosis of trichomoniasis in pigeons

Document Type : Original Article

Authors

1 Graduated with a doctorate in veterinary medicine,Islamic Azad University, Shushtar-Iran

2 Assistant Professor, Department of Veterinary Medicine, Faculty of Veterinary Medicine, Shooshtar-Iranian

Abstract

Trichomoniasis is one of the common infectious diseases in birds, which is caused by the flagellated protozoan Trichomonas gallinae. The symptoms of the disease are various and include anorexia, diarrhea, swallowing, purulent plaques and bad and sour smell inside of the oropharyngeal cavity. Conventional diagnosis is based on the preparation of a wet mount and its examination under a microscope. This is a descriptive-analytical study. Since no study has been conducted to compare the wet mount method and PCR in the diagnosis of trichomoniasis in Tehran, In this study, we decided to use the molecular PCR method and based on the amplification of the ITS1/5.8S/ITS2 gene of Trichomonas gallinae to compare its accuracy, specificity and sensitivity with the wet mount method. From 60 pigeons referred to the clinics in Tehran, 2 samples were taken from their oral cavity and crop using sterile swab. A wet mount was quickly prepared from a sample and examined under a microscope. Another sample was also used for PCR testing. 40% (24 samples) with the wet mount method and 85% (51 samples) were positive with the PCR method. The data was analyzed with SPSS software version 24 and with a confidence level of 95% there is a significant difference between the results of wet mount and PCR in the diagnosis of trichomoniasis. Therefore, compared to PCR, the wet mount method has good "specificity" in the diagnosis of trichomoniasis, while it has low "sensitivity" and "accuracy" in the diagnosis of trichomoniasis.

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Journal of Veterinary Laboratory Research
Volume 14, Issue 2 - Serial Number 22
February 2023
Pages 163-169

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