Isolation of pathogenic agents of silkworms and some its pathological aspectsin Guilan province

Nabian, Sedigheh; Iranmanesh, Fakhri; Khosravi, Ali Reza; Zahraei-Salehi, Taghi; Moradi, Mostafa

doi:10.22075/jvlr.2017.794

Isolation of pathogenic agents of silkworms and some its pathological aspectsin Guilan province

Authors

Sedigheh Nabian

Fakhri Iranmanesh

Ali Reza Khosravi

Taghi Zahraei-Salehi

Mostafa Moradi

10.22075/jvlr.2017.794

Abstract

AbstractÂ There is a lack of Survey of silkworm diseases in Iran. Guilan province is the largest industry center sericulture in Iran. It seemed that study of sericultuers diseases in this province is necessary for prevention of diseases and decreasing the related economic loss. Â Â Specimens including sick and dead larves were collected from 92 sericultuers of Guilan province randomly, in spring 1385. Samples were tested for parasitic , viral, bacterial and fungal agents,Â using complementary tests including biochemical tests and specific staining.Â Using direct microscopic examination, in wet mount prepared from intestine wall, protozoa spores belong to Microsporidia phylum were observed in eight sericultuers which was not Nosema bombysis. Many polyhedra forms in hemolynph ,were diagnosed as the agent of Polyhedrosis or Grasserie disease( NPV) in %31.5 of sericultuers . The most important prevalent infection was bacterial agents including: Staphylococcus aureus, and S.epidermidis as septicemia diseases in %95/6 of sericultuers. Fungal agents were diagnosed in 26 of sericultuers (%28/2) containing: Aspergillus fumigatousÂ ( %11/5) and Beauveria bassiana or Red Muscardin in %3/8 of sericultuers. Â

Keywords

Keywords: infection

Silkworm

Guilan