Histomorphometric evaluation of the effect of L-Carnitine on testicular injuries caused by ethanol in rats

Document Type : Original Article

Authors

1 Graduated from veterinary doctorate, Faculty of Veterinary Medicine, Semnan University

2 Department of Basic Science, Faculty of Veterinary medicine, Semnan University, Semnan, Iran.

3 Department of Clinical science, Semnan University, Semnan, Iran

4 Department of Basic Sciences, Faculty of Veterinary Medicine, Semnan University

Abstract

In this study, the effect of L-Carnitine was investigated on reducing the alcohol damage in the testes of adult rats. Alcohol leads to oxidative damage and L-Carnitine protects from oxidative damage as an antioxidant. In this study, in 20 adult male Wistar rats damage were induced by 30% ethanol with a dose of three gr/kg. To prevent injury, L-Carnitine with a dose of 100mg/kg was used. Finally, blood samples were taken from the rats and testosterone concentration was measured. After the rats euthanizing, the testes were removed, weighed and fixed in 10% formalin buffer solution for 72 hours. After processing with standard histological methods and paraffin molding, 5 microns sections were prepared and studied histomorphometrically after H&E staining. Findings of this study revealed that Ethanol significantly reduced testicular weight, serum testosterone concentration, and testicular histomorphometric parameters. Treatment of L-Carnitine resulted in a significant increase in testicular weight, seminiferous tubule diameter, epithelial thickness, serum testosterone concentration and number of Spermatogonia, Primary Spermatocytes, Spermatids and Leydig cell compared to the positive group. Therefore, it can be concluded that oral L-Carnitine at a dose of 100 mg/kg can partially prevent the damage caused by ethanol.

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Journal of Veterinary Laboratory Research
Volume 13, Issue 2 - Serial Number 20
September 2021
Pages 137-149

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