Investigation of the prevalence and antibiotic susceptibility of Brucella melitensis in dairy products supplied in Garmsar County

Document Type : Original Article

Authors

1 Shahrekord Azad University, Faculty of Veterinary Medicine, Department of Food Quality Control Health

2 Professor, Food Hygiene Department, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abstract

Raw milk contaminated with Brucella species is one of the most important ways of transmitting brucellosis to humans. The aim of the present study was to investigate the prevalence and antibiotic susceptibility of Brucella melitensis in dairy products sold in Garmsar city. In this study, 180 dairy samples including raw Milk cow, sheep, goat and buffalo, traditional cheese, and traditional yogurt sold in Garmsar city were sampled and transferred to the laboratory under cold conditions. The samples were detected by MRT test, streak culture, and Multiplex PCR, and their antibiotic resistance was determined by the Kirby-Bauer disk diffusion method. The results showed that the MRT method determined the infection rate of Brucella melitensis to be 11.11%, streak culture to be 22.22%, and PCR to be 7.78%. The frequency of virulence genes was bvfA in 9 samples (64.25%), virfB in 5 samples (35.74%), and ure in 3 samples (21.42%). Antibiotic resistance showed that the highest resistance was to tetracycline (92.5%) and ampicillin (82.5%) and the lowest resistance was to rifampin and imipenem (0%). According to the results obtained, it can be concluded that the consumption of raw and traditional dairy products can be a source of transmission of malt fever to humans and the use of traditional and unpasteurized dairy products should be prohibited.
 
 

Keywords

Main Subjects

References

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Journal of Veterinary Laboratory Research
Volume 16, Issue 2 - Serial Number 26
February 2025
Pages 131-141

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