Genomic investigation of Toxoplasma gondii in sheep milk collected from Sistan region by PCR method

Document Type : Original Article

Authors

1 Graduated student of parasitology , Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

3 Laboratory expert, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

Abstract

Toxoplasmosis is a common disease in humans and other animals. Among animals, sheep and goats are more infected with Toxoplasma gondii. Humans can be infected with in various ways; Toxoplasmosis is usually transmitted to humans through consumption of raw or undercooked meat infected with Toxoplasma cysts, unpasteurized milk, contact with infected cats, or water and food contaminated with cat oocysts. If a person is exposed to toxoplasmosis in the first pregnancy, there is a possibility that the fetus will also be infected. Considering the great importance of Toxoplasma gondii as a possible agent transmitted through milk, the aim of this study is molecular investigation of toxoplasmosis in sheep milk samples collected from Sistan region. In this research, the polymerase chain reaction (PCR) method was used to detect Toxoplasma gondii DNA by examining the B1 gene in sheep milk. In the present study, 90 raw sheep milk from different regions were evaluated in the laboratory.In this way, the DNA extracted from the samples and the parasite was tracked using the polymerase chain reaction. The results of this study showed that 2 samples (2.2%) were infected with Toxoplasma gondii. The presence of Toxoplasma gondii DNA in sheep's milk increases the possibility of transmission of this parasite through consumption of raw milk. Therefore, proper pre-consumption of milk is recommended to reduce the possibility of toxoplasma gondii.

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Journal of Veterinary Laboratory Research
Volume 16, Issue 1 - Serial Number 25
September 2024
Pages 39-45

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