Genomic investigation of Toxoplasma gondii in sheep milk collected from Sistan region by PCR method

Document Type : Original Article

Authors

1 Graduated student of parasitology , Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

3 Laboratory expert, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

Abstract

Toxoplasmosis is a common disease in humans and other animals. Among animals, sheep and goats are more infected with Toxoplasma gondii. Humans can be infected with in various ways; Toxoplasmosis is usually transmitted to humans through consumption of raw or undercooked meat infected with Toxoplasma cysts, unpasteurized milk, contact with infected cats, or water and food contaminated with cat oocysts. If a person is exposed to toxoplasmosis in the first pregnancy, there is a possibility that the fetus will also be infected. Considering the great importance of Toxoplasma gondii as a possible agent transmitted through milk, the aim of this study is molecular investigation of toxoplasmosis in sheep milk samples collected from Sistan region. In this research, the polymerase chain reaction (PCR) method was used to detect Toxoplasma gondii DNA by examining the B1 gene in sheep milk. In the present study, 90 raw sheep milk from different regions were evaluated in the laboratory.In this way, the DNA extracted from the samples and the parasite was tracked using the polymerase chain reaction. The results of this study showed that 2 samples (2.2%) were infected with Toxoplasma gondii. The presence of Toxoplasma gondii DNA in sheep's milk increases the possibility of transmission of this parasite through consumption of raw milk. Therefore, proper pre-consumption of milk is recommended to reduce the possibility of toxoplasma gondii.

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References

Amroabadi, M. A., Rahimi, E., & Shakerian, A. (2021). Study of the seasonal and geographical prevalence of Toxoplasma gondii in milk of ruminants by nested-PCR.
Armand, B., Solhjoo, K., Shabani-Kordshooli, M., Davami, M. H., & Sadeghi, M. (2016). Toxoplasma infection in sheep from south of Iran monitored by serological and molecular methods; risk assessment to meat consumers. Veterinary world, 9(8), 850.
Asgari, Q., Mikaali, F., Ahmadi, B., & Saleh, B. M. (2019). Effects of Lawsonia inermis Extract on Toxoplasma gondii in In-Vitro and In-Vivo Environments.
Asghari, A., Yousefi, A., & Majidiani, H. (2022). Toxoplasmosis and its current status in Iran: A narrative review. Journal of Rafsanjan University of Medical Sciences, 20(11), 1253-1278.
Boughattas, S. (2017). Toxoplasma infection and milk consumption: Meta-analysis of assumptions and evidences. Critical Reviews in Food Science and Nutrition, 57(13), 2924-2933.
da Silva, J. G., Alves, B. H. L., Melo, R. P. B., Kim, P. C. P., Neto, O. L. S., Bezerra, M. J. G., Sá, S. G., & Mota, R. A. (2015). Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in raw milk of sheep and goats of local breeds reared in Northeastern Brazil. Acta tropica, 142, 145-148.
Hunter, C. A., & Sibley, L. D. (2012). Modulation of innate immunity by Toxoplasma gondii virulence effectors. Nature Reviews Microbiology, 10(11), 766-778.
Inpankaew, T., Pinyopanuwut N Fau - Chimnoi, W., Chimnoi W Fau - Kengradomkit, C., Kengradomkit C Fau - Sununta, C., Sununta C Fau - Zhang, G., Zhang G Fau - Nishikawa, Y., Nishikawa Y Fau - Igarashi, I., Igarashi I Fau - Xuan, X., Xuan X Fau - Jittapalapong, S., & Jittapalapong, S. (2010). Serodiagnosis of Toxoplasma gondii infection in dairy cows in Thailand. (1865-1682 (Electronic)).
Jahantigh, F. F., Rasekh, M., Ganjali, M., & Sarani, A. (2020). Seroprevalence of Toxoplasma gondii infection among pregnant women and small ruminant populations in Sistan region, Iran.
Khamsian, E. M., Hajimohammadi, B., Eslami, G., Fallahzadeh, M. H., & Hosseini, S. S. (2021). Toxoplasma gondii in Milk of Human and Goat from the Desert Area in Central Iran. (1735-7020 (Print)).
Liu, Q., Wang, Z. D., Huang, S. Y., & Zhu, X. Q. (2015). Diagnosis of toxoplasmosis and typing of Toxoplasma gondii. (1756-((Electronic).
Meftahi, M., Mashak, Z., & Kefayat, M. (2021). Study the molecular prevalence rate of Toxoplasma gondii in raw milk samples collected from Alborz province by PCR method. Journal of Animal Environment, 13(3), 41-48.
Mostafavi, S. N., & Jalali Monfared, L. (2012). Toxoplasmosis epidemiology in Iran: a systematic review. Journal of Isfahan Medical School, 30.(176).
Ossani, R., Borges, H., Souza, A., Sartor, A., Miletti, L., Federle, M., & Moura, A. (2017). Toxoplasma gondii in milk of naturally infected dairy ewes on west mesoregion of Santa Catarina state, Brazil. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 69(05), 1294-1300.
Saad, N. M., Hussein, A. A., & Ewida, R. M. (2018). Occurrence of Toxoplasma gondii in raw goat, sheep, and camel milk in Upper Egypt. Veterinary world, 11(9), 1262.
Tavassoli, M., Esmaeilnejad, B., Malekifard, F., Soleimanzadeh, A., & Dilmaghani, M. (2013). Detection of Toxoplasma gondii DNA in Sheep and Goat Milk in Northwest of Iran by PCR-RFLP. Jundishapur Journal of Microbiology, 6 (10).
 
Journal of Veterinary Laboratory Research
Volume 16, Issue 1 - Serial Number 25
September 2024
Pages 39-45

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