Detection of Respiratory Syncytial Virus (RSV) Ag in Pneumonic Lambs Histopathological and Immunohistochemical Study

Document Type : Original Article

Author

Azad University Road

Abstract

Respiratory Syncytial Virus (RSV) due to induction of respiratory system infections causes significant economic losses to livestock rearing industry. In the present study, in which the main goal was to determine the prevalence of (RSV) infection in lambs slaughtered in Garmsar slaughterhouse, a total of 3010 lambs were subjected to postslaughter inspection. Among them, 137 (4.55%) cases were found having macroscopic lesions of pneumonia particularly on the cranioventral lobs. Lesions were more frequently encountered in anteroventral lobes than caudal lobes. Lung with macroscopic lesions of pneumonia were sampled and Formalin-fixed and paraffin-embedded lung tissue samples were processed histopathologically (H&E technique) and immunohistochemically stained using routine avidin-biotin-peroxidase complex techniques to detect (RSV) antigens. In the present study, out of 137 cases with pneumonia, in 5 cases (3.6%) (RSV) viral antigens was detected, which were mainly found in the cytoplasm of bronchiolar epithelial cells, type II pneumocytes, and less frequently in the epithelial cells of bronchial glands, syncytial cells, alveolar macrophages, and lymphocytes and plasma cells. In conclusion, despite of the determining the low prevalence of (RSV) viral antigens in pneumonic lungs of lambs in Garmsar county, but a possible role of (RSV) in the induction of interstitial pneumonia for the first time in lambs in this region of the country was suggested.
 

Keywords

Main Subjects

References

Baker Jc Fau - Frey, M. L., & Frey, M. L. (1985). Bovine respiratory syncytial virus. (0749-0720 (Print)).
Bancroft, J. D., & Gamble, M. (2008). Theory and practice of histological techniques. Elsevier health sciences.
Belknap, E. B., Ciszewski Dk Fau - Baker, J. C., & Baker, J. C. (1986). Experimental respiratory syncytial virus infection in calves and lambs. (1040-6387 (Print)).
Belknap, E. B., Ciszewski Dk Fau - Baker, J. C., & Baker, J. C. (1995). Experimental respiratory syncytial virus infection in calves and lambs. (1040-6387 (Print)).
Bryson, D. G., Evermann Jf Fau - Liggitt, H. D., Liggitt Hd Fau - Foreyt, W. J., Foreyt Wj Fau - Breeze, R. G., & Breeze, R. G. (1988). Studies on the pathogenesis and interspecies transmission of respiratory syncytial virus isolated from sheep. (0002-9645 (Print)).
Caswell, J., & Williams, K. (2007). Respiratory System In: Maxie MG, editor. Jubb, Kennedy & Palmer’s Pathology of Domestic Animals. 5th ed Philadelphia: Elsevier Ltd, 591-593.
Ceribasi, S., Ozkaraca, M., Ozer, H., & Ceribasi, A. (2013). Diagnosing respiratory syncytial virus using immunofluorescence and immunohistochemistry methods in caprine lungs with bronchopneumonia.
Duncan Jr, R. B., & Potgieter, L. N. (1993). Antigenic diversity of respiratory syncytial viruses and its implication for immunoprophylaxis in ruminants. Veterinary microbiology, 37(3-4), 319-341.
Eleraky, N. Z., Kania, S. A., & Potgieter, L. N. (2001). The ovine respiratory syncytial virus F gene sequence and its diagnostic application. Journal of veterinary diagnostic investigation, 13(6), 455-461.
Forghani, B. (2016). Diagnosis by viral antigen detection. In Lennette's Laboratory Diagnosis of Viral Infections (pp. 113-132). CRC Press.
Gülbahar, Y., Çabalar, M., & Ertürk, A. (2002). Detection by immunoperoxidase technique of parainfluenza type-3 virus and respiratory syncytial virus antigens in naturally occurring pneumonia in lambs. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 13, 74-77.
Gurcay, M., & Bolat, Y. (2019). Serological survey of Respiratory Syncytial Virus (RSV) infections of sheep in Elazig and it's vicinity. The Journal of Health Sciences of Firat University (Turkey), 10(2).
Haines, D. M., Clark Eg Fau - Chelack, B. J., & Chelack, B. J. (1989). The detection of bovine respiratory syncytial virus in formalin fixed bovine lung with commercially available monoclonal antibodies and avidin biotin complex immunohistochemistry. (0830-9000 (Print)).
Jamshidi, K., & Ozmen, O. (2019). Immunohistochemical study on Respiratory Syncytial Virus antigen (RSV) in Pneumonic Goat Lungs.
Masot, A., Gomez, L., Martînez, M., Gazquez, A., & Redondo, E. (1993). Experimental infection of lambs with bovine respiratory syncytial virus: pathological and haematological studies. Revue de Medecine Veterinaire (France), 144, (10).
Trahman, T., & Singh, B. (1990). Incidence and pathology of viral pneumonia in goats.
Wellemans, G. (2013). Bovine respiratory syncytial virus. Virus infections of ruminants, 363.
 Tscherne, A. (2021). Characterization of a recombinant Modified Vaccinia virus Ankara expressing the severe acute respiratory syndrome virus 2 spike protein lmu.
Journal of Veterinary Laboratory Research
Volume 16, Issue 1 - Serial Number 25
September 2024
Pages 25-32

Files

Share

How to cite

Statistics