Detection of Respiratory Syncytial Virus (RSV) Ag in Pneumonic Lambs Histopathological and Immunohistochemical Study

Document Type : Original Article

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Azad University Road

Abstract

29




 



Respiratory Syncytial Virus (RSV) due to induction of respiratory system infections causes significant economic losses to livestock rearing industry. In the present study, in which the main goal was to determine the prevalence of (RSV) infection in lambs slaughtered in Garmsar slaughterhouse, a total of 3010 lambs were subjected to postslaughter inspection. Among them, 137 (4.55%) cases were found having macroscopic lesions of pneumonia particularly on the cranioventral lobs. Lesions were more frequently encountered in anteroventral lobes than caudal lobes. Lung with macroscopic lesions of pneumonia were sampled and Formalin-fixed and paraffin-embedded lung tissue samples were processed histopathologically (H&E technique) and immunohistochemically stained using routine avidin-biotin-peroxidase complex techniques to detect (RSV) antigens. In the present study, out of 137 cases with pneumonia, in 5 cases (3.6%) (RSV) viral antigens was detected, which were mainly found in the cytoplasm of bronchiolar epithelial cells, type II pneumocytes, and less frequently in the epithelial cells of bronchial glands, syncytial cells, alveolar macrophages, and lymphocytes and plasma cells. In conclusion, despite of the determining the low prevalence of (RSV) viral antigens in pneumonic lungs of lambs in Garmsar county, but a possible role of (RSV) in the induction of interstitial pneumonia for the first time in lambs in this region of the country was suggested.
 

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Journal of Veterinary Laboratory Research
Volume 16, Issue 1 - Serial Number 25
September 2024
Pages 29-38

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