Investigation of the Prevalence Rate of Giardiasis at Livestocks in Khorramabad

Document Type : Original Article

Authors

1 Department of Veterinary, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran.

2 Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran.

3 Young researchers and Elite club, Babol Branch, Islamic Azad University, Babol, Iran.

Abstract

Giardia parasite is one of the most important causes of digestive problems in humans and animals The purpose of this research; The investigation of the current situation was the prevalence of giardiasis in the cattle farms of Khorram Abad city. The present survey was conducted on 200 cows and in 6 cattle farms around the city of Khorram Abad (these six cattle farms were located in Khorram Abad), between May and August of 2022. The stool sample was obtained directly from the rectum using a disposable glove, and after turning the glove over itself and closing it with paper tape, the cow's ear number was written on the tape. Then the samples were taken to the laboratory and kept at the temperature of the refrigerator. In each case, the consistency of feces (watery, loose, normal and hard) and the physical condition of the animal (thin, normal and fat) along with its age and gender were recorded in the relevant forms. Zinc sulfate solution was used for flotation of Giardia cysts. After staining with trichrome kit, the resulting slides were examined using an optical microscope and it was observed that the prevalence of giardia in cows of Khorramabad city was 13.5% and this prevalence is affected by various factors, such as the age, sex, and method of keeping the animal. The level of contamination in males was higher than females, in young population more than old population, and in traditional maintenance method more than industrial type. Therefore, due to the significant prevalence of this protozoa, the application of management and health principles should be considered.
 

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Main Subjects

References

Ansell BR, McConville MJ, Ma'ayeh SY, Dagley MJ, Gasser RB, Svärd SG, et al. Drug resistance in Giardia duodenalis. Biotechnology advances. 2015;33(6):888-901.
Li S, Zou Y, Zhang X-L, Wang P, Chen X-Q, Zhu X-Q. Prevalence and multilocus genotyping of Giardia lamblia in cattle in Jiangxi Province, China: novel assemblage E subtypes identified. The Korean Journal of Parasitology. 2020;58(6):681.
Meng X-Z, Kang C, Wei J, Ma H, Liu G, Zhao J-P, et al. Meta-Analysis of the Prevalence of Giardia duodenalis in Cattle in China. Foodborne Pathogens and Disease. 2023;20(1):17-31.
Ryan U, Hijjawi N, Feng Y, Xiao L. Giardia: an under-reported foodborne parasite. International Journal for Parasitology. 2019;49(1):1-11.
Dixon B. Transmission dynamics of foodborne parasites on fresh produce.  Foodborne parasites in the food supply web: Elsevier; 2015. p. 317-53.
Squire SA, Yang R, Robertson I, Ayi I, Ryan U. Molecular characterization of Cryptosporidium and Giardia in farmers and their ruminant livestock from the Coastal Savannah zone of Ghana. Infection, Genetics and Evolution. 2017;55:236-43.
Fantinatti M, Bello AR, Fernandes O, Da-Cruz AM. Identification of Giardia lamblia assemblage E in humans points to a new anthropozoonotic cycle. The Journal of infectious diseases. 2016;214(8):1256-9.
Jian Y, Zhang X, Li X, Karanis G, Ma L, Karanis P. Prevalence and molecular characterization of Giardia duodenalis in cattle and sheep from the Qinghai-Tibetan Plateau Area (QTPA), northwestern China. Veterinary parasitology. 2018;250:40-4.
Zhao Z-Y, Li M-H, Lyu C, Meng X-Z, Qin Y-F, Yang X-B, et al. Prevalence of Giardia duodenalis among dogs in China from 2001 to 2021: a systematic review and Meta-analysis. Foodborne Pathogens and Disease. 2022;19(3):179-91.
Geng H-L, Yan W-L, Wang J-M, Meng J-X, Zhang M, Zhao J-X, et al. Meta-analysis of the prevalence of Giardia duodenalis in sheep and goats in China. Microbial Pathogenesis. 2023;179:106097.
Tovar J, León-Avila G, Sánchez LB, Sutak R, Tachezy J, Van Der Giezen M, et al. Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation. Nature. 2003;426(6963):172-6.
Mahato M, Singh D, Rana H, Acharya K. Prevalence and risk factors associated with Giardia duodenalis infection in dairy cattle of Chitwan, Nepal. Journal of parasitic diseases. 2018;42(1):122-6.
Wang H, Zhao G, Chen G, Jian F, Zhang S, Feng C, et al. Multilocus genotyping of Giardia duodenalis in dairy cattle in Henan, China. PloS one. 2014;9(6):e100453.
Azcona-Gutiérrez JM, de Lucio A, Hernández-de-Mingo M, García-García C, Soria-Blanco LM, Morales L, et al. Molecular diversity and frequency of the diarrheagenic enteric protozoan Giardia duodenalis and Cryptosporidium spp. in a hospital setting in Northern Spain. PloS one. 2017;12(6):e0178575.
Zhang XX, Tan QD, Zhao GH, Ma JG, Zheng WB, Ni XT, et al. Prevalence, risk factors and multilocus genotyping of Giardia intestinalis in dairy cattle, Northwest China. Journal of Eukaryotic Microbiology. 2016;63(4):498-504.
Park Y-J, Cho H-C, Jang D-H, Park J, Choi K-S. Multilocus genotyping of Giardia duodenalis in pre-weaned calves with diarrhea in the Republic of Korea. Plos one. 2023;18(1):e0279533.
Kılınç ÖO, Ayan A, Çelik BA, Çelik ÖY, Yüksek N, Akyıldız G, et al. The Investigation of Giardiasis (Foodborne and Waterborne Diseases) in Buffaloes in Van Region, Türkiye: First Molecular Report of Giardia duodenalis Assemblage B from Buffaloes. Pathogens. 2023;12(1):106.
Hailu M, Asmare K, Gebremedhin EZ, Sheferaw D, Gizaw D, Di Marco V, et al. Cryptosporidium and Giardia infections in dairy calves in southern Ethiopia. Parasite epidemiology and control. 2020;10:e00155.
Malekifard F, Ahmadpour M. Molecular detection and identification of Giardia duodenalis in cattle of Urmia, northwest of Iran. Veterinary Research Forum. 2018;9(1):81.
Mendonça C, Almeida A, Castro A, de Lurdes Delgado M, Soares S, da Costa JMC, et al. Molecular characterization of Cryptosporidium and Giardia isolates from cattle from Portugal. Veterinary parasitology. 2007;147(1-2):47-50.
Rayani M, Unyah NZ, Hatam G. Molecular identification of Giardia duodenalis isolates from Fars province, Iran. Iranian journal of parasitology. 2014;9(1):70.
Fallah E, Nahavandi KH, Jamali R, Poor BM, Asgharzadeh M. Molecular identification of Giardia duodenalis isolates from human and animal reservoirs by PCR-RFLP. Journal of Biological Sciences. 2008;8(5):896-901.
Tappeh KH, Manafi G, Asgharzadeh M, Manafi F. Incidence of Giardia lamblia subspecies by PCR-RFLP in Stool specimens of hospitalized children at Urmia Mutahhari hospital, West Azerbaijan province, Iran. Iranian journal of parasitology. 2014;9(4):541.
Journal of Veterinary Laboratory Research
Volume 15, Issue 2 - Serial Number 24
September 2023
Pages 167-179

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