Phylogenetic study of Hepatozoan canis blood parasite in stray dogs of Guilan province

Document Type : Original Article

Authors

1 Department of Biology, Faculty of Science, University of Guilan, Rasht, IRan

2 Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran

Abstract

Reports in the literature indicate that species of Hepatozoon canis blood parasites are common in Iranian dog populations. In this study, we aimed to investigate the prevalence of H. canis infections and their phylogenetic relationships in dogs from an animal shelter in Guilan province using polymerase chain reaction (PCR) and sequence analysis.A total of 20 blood samples were subjected to PCR to amplify a fragment of 562 bp from the 18S rRNA gene of Hepatozoonspp. The PCR results showed that the Hepatozoon infection rate in the dogs was 10%. (2/20). Sequence analysis revealed that the H. canis found in this study was 100% matched to H. canisin in the NCBI GenBank database, which originated from a dog in Tehran (accession number KX880505) and a golden jackal in Romania (accession number KX712126). In the phylogenetic analysis, these three sequences are grouped in the same clade. This was the first study of dogs from Guilan province using molecular phylogenetic methods. There is a need for more comprehensive studies to determine the infection status in Iran.
 

Keywords

Main Subjects

References

Amoli, A., Khoshnegah, A.R., Razmi, G.R. 2012. A Preliminary Parasitological Survey of Hepatozoon spp. Infection in Dogs in Mashhad, Iran. Iranian Journal of Parasitology. 7 (4), 99-103.
Aktas, M. 2014. A survey of ixodid tick species and molecular identification of tick-borne pathogens. Veterinary Parasitology. 200, 276–283.
Almazán, C., Scimeca R.C., Reichard, M.V.,  Mosqueda, J. 2022. Babesiosis and Theileriosis in North America. Pathogens. 11, 168. https://doi.org/10.3390/pathogens11020168.
Aydin, M.F., Sevinc, F.,  Sevinc, M. 2015. Molecular detection and characterization of Hepatozoon spp. in dogs from central part of Turkey. Tick Borne Disease. 6, 388-392. DOI: 10.1016/j.ttbdis.2015.03.004.  
Baneth, G., Mathew, J.S., Shkap, V., Macintire, D.K., Barta, J.R., Ewing, S.A. 2003. Canine hepatozoonosis: Two disease syndromes caused by separate Hepatozoon spp. Trends Parasitology. 19, 27–31.
Dalimi, A., Jameie, F., Mohammadiha, A., Barati, M.,  Molaei, S. 2017. Molecular detection of Hepatozoon canis in dogs of Ardabil Province, Northwest of Iran. Archive of Razi Instute,.72(3),197-201.
Davies, A.J., Johnston, M.R.L. 2000. The biology of some intraerythrocytic parasites of fishes, amphibians and reptiles. Advances in Parasitology. 45, 1–107.
Gondim, L.F., Kohayagawa, A., Alencar, N.X, Biondo, A.W., Takahira, R.K.,  Franco, S.R. 1998. Canine hepatozoonosis in Brazil: description of eight naturally occurring cases. Vetrinary Parasitology. 74(24):319–323. doi: 10.1016/S0304-4017(96)01120-X.
Khoshnegah, J.,  Mohri, M., Movassaghi, A.Z.,  Mehrjerdi, H.K. 2009. A Preliminary Parasitological Survey of Hepatozoon Spp. Infection in Dogs in Mashhad, Iran. Iranian Journal of Parasitology. 7(4), 99-103.
Li, W., Yang, Y., Liu, Z.H., Zhao, Y.J., Zhang, Q., Zhang, L., Cheung, T.,  Xiang, Y.T. 2020. Progression of mental health services during the COVID-19 outbreak in China. International Journal of Biological Sciences. 16(10), 1732–1738. https://doi.org/ 10.1111/inm.12824.
Maia, J.P., Harris, D.J., Perera, A. 2011. Molecular survey of Hepatozoon species in lizards from North Africa. Journal Parasitology. 97, 513–517.
Netherlands, E.C.,Stroebel, C., Louis, H., Preez, D., Shabangu, N., Matjila, T., Schalkwy, L.V ., Penzhorn, B.L. 2021. Molecular confirmation of high prevalence of species of Hepatozoon infection in free-ranging African wild dogs (Lycaon pictus) in the Kruger National Park, South Africa. International Journal for Parasitology: Parasites and Wildlife. 4, 335-340.
Otranto, D., Dantas-Torres, F., Weigl, S., Latrofa, M. S., Stanneck, D., Decaprariis, D., Capelli, G., Baneth, G. 2011. Diagnosis of Hepatozoon canis in young dogs by cytology and PCR. Parasite &Vectors. 4, 55.
Peirce, M., Laurenson, M., Gascoyne, S. 1995. Hepatozoonosis in cheetahs and wild dogs in the Serengeti ecosystem. African Journal of Ecology. 33, 273–275.
Rubini, A.S., Mundim, A.V., Eyal, O., Talmi-Frank, D., Cury, M.C.,  Baneth, G. 2014. Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil, Research in Veterinary Science. 97, 326-329.
Sevilla, E., González, L.M.,  Luque, D.,  Gray, J.,  Montero, E. 2018. Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy. Scientific Reports. 8, 14116 | DOI:10.1038/s41598-018-32349-7.
Srisutham, S., Saralamba, N.T., Malleret, B., Renia, L. , Dondorp, A.D., Imwong, M. 2017. Four human Plasmodium species quantification using droplet digital PCR. PLoS ONE. 12(4), e0175771. https://doi.org/10.1371/journal.pone.0175771.
Soltani , R., Dalimi, A. 2018. A Molecular Study on Hepatozoon canis Infection in Dogs in Tehran (Iran). Archives of Razi Institute. 73(4), 257-263.
Valkiūnas, G Ilgunas, M., Bukauskaite, D.,Iezhova, T.A. 2016. Description of Haemoproteus  ciconiae sp. nov. (Haemoproteidae, Haemosporida) from the white stork Ciconia ciconia, with remarks on insensitivity of established polymerase chain reaction assays to detect this infection. Parasitology Research. 115, 2609–2616. Doi 10.1007/s00436-016-5007-4.
Williams, B.M., Berentsen, A., Shock, B.C., Teixiera, M.R., Dunbar, M.R., Becker, M.S., Yabsley, M.J. 2014. Prevalence and diversity of Babesia, Hepatozoon, Ehrlichia, and Bartonella in wild and domestic carnivores from Zambia, Africa. Parasitology Research. 113, 911–918.

Zeinali1, M.R., Malekifard, F., Rakhshanpour, A., Yakhchali, M. 2022. Study on Hepatozoon canis in dogs in Urmia region. Iranian Journal of Animal Science. 52, (4), 253-260. DOI: 10.22059/ijas.2021.329716.653845. In Persian.

 
Journal of Veterinary Laboratory Research
Volume 15, Issue 2 - Serial Number 24
September 2023
Pages 121-129

Files

Share

How to cite

Statistics