Investigating the resistance to common antiseptics, Behsadin, Titmate and Damosib in Pseudomonas aeruginosa isolates isolated from mastitis milk of Shiraz and Kazerun cows.

Document Type : Original Article

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1- Assistant Professor, Department of Microbiology, Department of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University, Kazerun Branch, Kazerun, Iran

Abstract

The use of breast disinfectants is one of the tools to control the spread of factors involved in mastitis infection, especially Pseudomonas aeruginosa.In this research, 100 samples of bovine mastitis milk were collected from Fars province and Kazerun city, of which 88 were environmental strains and 12 were clinical strains, of which; 8 strains were identified as P. aeruginosa from bovine mastitis milk samples in Shiraz and Kazerun regions. Then the antibiogram test was performed to determine the minimum inhibitory concentration (MIC) using three disinfectants, Titmit, Behsadin, and Damosib, based on the microdilution method.Results: Pseudomonas aeruginosa strains were 37.5% sensitive and 62.5% resistant, 36% environmental and 64% clinical. In examining the difference between the mean MIC changes of three disinfectants based on the Mann-Whitney U Test, the difference of Behsadin disinfectant MIC changes with other disinfectants was significant(P≤0.05) the same way, the difference of MIC changes of Titmate disinfectant with two other disinfectants was highly significant(P≤0.01). The results obtained from the research showed that the concentrations effective in inhibiting the growth of the strains are much lower than the amount recommended by the manufacturing companies. It is believed that the high use of these antimicrobial substances may cause selective pressure and lead to the growth of strains resistant to disinfectants. Also, the efflux pumps involved in resistance, despite the expectation, did not play an effective role in creating resistance in Pseudomonas aeruginosa strains, and they confirmed the hypothesis that the resistance created in the studied strains was probably due to other mechanisms.

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