بررسی مقاومت جدایه های سودوموناس ائروژنز جدا شده از شیرهای ورم پستانی گاو نسبت به ضدعفونی کننده های داموسیب،تیت میت و بهسادین

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار گروه میکروبیولوژی،گوه دامپزشکی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی، واحد کازرون،کازرون، ایران

2 فارغ التحصیل دکترای حرفه ای دامپزشکی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی، واحد کازرون، کازرون، ایران

3 استادیار گروه انگل شناسی، گروه دامپزشکی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی، واحد کازرون، کازرون، ایران

4 مربی گروه فیزیولوژی، گروه دامپزشکی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی، واحد کازرون، کازرون، ایران

چکیده

استفاده از ضدعفونی کننده های پستانی یکی از ابزارهای کنترل شیوع عوامل دخیل در عفونت ورم پستان به ویژه سودوموناس ائروژنز است. هدف از این مطالعه تعیین میزان مقاومت سودوموناس ائروژنز به ضدعفونی کننده های رایج در دامداری ها نطیر تیت میت، بهسادین و داموسیب بود.
ﺩﺭ ﺍﻳﻦ ﭘﮋﻭﻫﺶ، 100 نمونه از شیر از گاوهای مبتلا به ورم پستان در شهرهای شیراز و کازرون جمع آوری شداز این تعداد؛ 8 باکتری جدا شده‌ از نمونه‌های‌ شیر ورم پستانی گاوان منطقه شیراز و کازرون‌ به‌ عنوان سودوموناس ائروژنز تشخیص‌ هویت داده شدند. سپس آزمایش تعیین حداقل غلظت مهار کنندگی( MIC) با استفاده از سه ضدعفونی کننده انجام شد.در بررسی اختلاف تغییرات MIC سه ضدعفونی کننده براساس آزمون Mann-Whitney U test بهسادین با ضدعفونی کننده های دیگر معنادار بود(P<0.05)، به همین ترتیب اختلاف تغییرات MIC ضدعفونی کننده تیت میت با دو ضدعفونی کننده دیگر قویا معنادار بود(P<0.01).نتایج به دست آمده از تحقیق نشان دادند غلظت‌های موثر بر مهاررشد سویه ها بسیار کمتر از میزان توصیه شده توسط شرکت‌های سازنده است. اعتقاد بر این است که استفاده زیاد از این مواد ضد میکروبی ممکن است باعث فشار انتخابی شده و به رشد گونه‌های مقاوم در برابر ضد عفونی کننده ها منجر شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigating the resistance to common antiseptics, Behsadin, Titmate and Damosib in Pseudomonas aeruginosa isolates isolated from mastitis milk of Shiraz and Kazerun cows.

نویسندگان [English]

  • hossein fattahi 1
  • Sajad Shekari 2
  • Ahad olyaei 3
  • Fatemeh Jouibar 4
1 1- Assistant Professor, Department of Microbiology, Department of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University, Kazerun Branch, Kazerun, Iran
2 Doctor of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University. Kazerun Branch.Kazerun.Iran.
3 Assistant Professor in Department of parasitology, Faculty of Veterinary Medicine, Islamic Azad University. Kazerun Branch.Kazerun.Iran.
4 instructor in Department of physiology, Faculty of Veterinary Medicine, Islamic Azad University. Kazerun Branch.Kazerun.Iran
چکیده [English]

The use of breast disinfectants is one of the tools to control the spread of factors involved in mastitis infection, especially Pseudomonas aeruginosa.In this research, 100 samples of bovine mastitis milk were collected from Fars province and Kazerun city, of which 88 were environmental strains and 12 were clinical strains, of which; 8 strains were identified as P. aeruginosa from bovine mastitis milk samples in Shiraz and Kazerun regions. Then the antibiogram test was performed to determine the minimum inhibitory concentration (MIC) using three disinfectants, Titmit, Behsadin, and Damosib, based on the microdilution method.Results: Pseudomonas aeruginosa strains were 37.5% sensitive and 62.5% resistant, 36% environmental and 64% clinical. In examining the difference between the mean MIC changes of three disinfectants based on the Mann-Whitney U Test, the difference of Behsadin disinfectant MIC changes with other disinfectants was significant(P≤0.05) the same way, the difference of MIC changes of Titmate disinfectant with two other disinfectants was highly significant(P≤0.01). The results obtained from the research showed that the concentrations effective in inhibiting the growth of the strains are much lower than the amount recommended by the manufacturing companies. It is believed that the high use of these antimicrobial substances may cause selective pressure and lead to the growth of strains resistant to disinfectants. Also, the efflux pumps involved in resistance, despite the expectation, did not play an effective role in creating resistance in Pseudomonas aeruginosa strains, and they confirmed the hypothesis that the resistance created in the studied strains was probably due to other mechanisms.

کلیدواژه‌ها [English]

  • Mastitis
  • Pseudomonasaeruginosa
  • disinfectants
  • Behsadin
  • Titmate
  • Damosib
Afsharivari, S, Sial, R, Kayahan Ch, Ishil F. The effect of efflux pump inhibitors on the minimum inhibitory concentration of benzalkonium chloride and chlorhexidine in Acinetobacter baumannii isolates isolated from patients referred to Gazi Hospital, Ankara. Urmia Medical Journal.5(27).21-25. (In Persian)
Dostundi, S., Abiri R, Mohajeri P, Alwandi Ah. Phenotype and genotype of efflux pumps in isolates of Acinetobacter species isolated from patients hospitalized in Imam Reza and Taleghani hospitals of Kermanshah. 2012. Journal of Mazandaran University of Medical Sciences. 126(25).1-11. (In Persian)
Japoninejad A, Sufian M, Ghaznavi Rad E. Molecular detection of ABC efflux pump genes in clinical strains of Acinetobacter baumannii and evaluation of its role in creating resistance to imipenem. Journal of Southern Medicine. 2013.Volume 17, Number 5, 408 to 415. (In Persian) Larry. A., Mohammadi. E., Masjidyan. F., Mahmoudian. M. Investigating the synergistic effect of noscapine with ofloxacin in Enterobacteriaceae resistant to fluoroquinolones. Scientific-Research Quarterly Journal of Iranian Medicinal and Aromatic Plants Research. 1387; 24(1): 94-100. (In Persian)Adewoye L, Sutherland A, Srikumar R, Poole K. The mexR repressor of the mexAB-oprM multidrug efflux operon in Pseudomonas aeruginosa: characterization of mutations compromising activity. J Bacteriol 2002; 184:4308-12.Ali et al. Association between antibiotics and disinfectants resistance profiles among Acinetobacter baumannii isolates in Zagazig university hospitals intensive care unit. Life Science Journal 2014;11(10) (ISSN:1097-8135). Azadpour et al, Presence of qacEΔ1 and cepA genes and susceptibility to a hospital biocide in clinical isolates of Klebsiella pneumoniae in Iran, Tropical Biomedicine, 01 Mar 2015, 32(1):109-115, PMID: 25801259 Bahador A, Taheri M. Medical Midrobiology.2nd ed. tehran: Ketabkhane farhang; 1389.Bialvaei AZ et al. Epidemiology of multidrug-resistant Acinetobacter baumannii strains in Iran: a systematic review and meta-analysis. J Chemother. 2017 Dec;29(6):327-337. Doi: 10.1080/1120009X.2017.1338377. Epub 2017 Jun 16. Biswas D, Tiwari M, Tiwari V. Comparative mechanism-based study on disinfectants against multidrug resistant Acinetobacter baumannii. J Cell Biochem. 2018 Aug 26. Doi: 10.1002/jcb.27373.Blot S. Limiting the attributable mortality of nosocomial infection and multidrug resistance in intensive care units. Clin Microbiol Infect 2008; 14(1): 5-13Bridier A, Briandet R, Thomas V, Dubois-Brissonnet F. Resistance of bacterial biofilms to disinfectants. Biofouling. 2011 Oct;27(9):1017-32. Doi: 10.1080/ 08927014.2011. 626899.. 79 Brooks G.F. Butel J.S. and Morse S.A. Jawets Melnick and Adelberg’s Medical Microbiology, Lange Basic Science. 2004; pp:262-267.Brown VI, Lowbury EJL. Use of an improved cetrimide agar medium and other culture methods for Pseudomonas aeruginosa. Journal of Clinical Pathology. 1965;18(6):752-6.Chapman JS. Biocide resistance mechanisms. International Biodeterioration & Biodegradation 51(2003) 133 – 138. Doi:10.1016/s0964-8305(02) 00097-5. Coyne S, Courvalin P, Perichon B. Efflux-Mediated Antibiotic Resistance in Acinetobacter spp. Antimicrobial Agents and Chemotherapy, Mar. 2011, p. 947–953.Doi:10.1128/AAC.01388-10. Daury L et al. Tripartite assembly of RND multidru efflux pumps. Nat Commun. 2016. 12;7:10731. Doi: 10.1038/ncomms10731. De Lorenzo V, Herrero M, Jakubzik U, Timmis KN. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. Journal of Bacteriology. 1990 Nov 1; 172(11):6568-72.Dijun Du et al. Multidrug efflux pumps: structure, function and regulation. Nature Reviews Microbiology, volume 16, pages523539(2018). DOI: 10.1038/s41579-018-0048-6. Lanjeri M, Younes-Cauet G, Oertel-Buchheit P, Porte D, Schnarr M, Granger-Schnarr M. A new LexA-based genetic system for monitoring andanalyzing protein heterodimerization in Escherichia coli. Molecular and General Genetics MGG. 2017Jan 1; 257(2):205-12.Driscoll JA, Brody SL, Kollef MH. Theepidemiology, pathogenesis and treatment of Pseudomonas aeruginosa infections. Drugs.2007 Feb 1;67(3):351-68.FAM Gomaa et al, High Prevalence of blaNDM-1, blaVIM, qacE, and qacED1 Genes and Their Association with Decreased Susceptibility to Antibiotics and Common Hospital Biocides in Clinical Isolates of Acinetobacter baumannii, Microorganisms, 5(2),18, doi:10.3390/microorganisms5020018 Fattahi.H, Diagnostic Veterinary Bacteriology. first ed. Norbakhshpress,1401Fendrich C. Halovibrio variabilis gen. nov. sp. nov., Pseudomonas halophila sp. nov. and a new halophilic aerobic coccoid Eubacterium from Great Salt Lake, Utah, USA. Systematic and Applied Microbiology. 1988;11(1):36-43.Fernandez L, Hancock REW. Adaptive and mutational resistance: role of porins      and efflux pumps in drug resistance. Clinical Microbiology Reviews 2012;25(4):661-81.Giamarellou H. Prescribing guidelines for serve Pseudomonas infections.      J Antimicrob Chemother. 2002; 49:229- 233.Gilbert P, mcbain AJ. Potential impact of increased use of biocides in consumer products on prevalence of antibiotic resistance. Clin Microbiol Rev. 2003; 16:189–208. PMID: 12692093. Gorgani N, Norozi, A Ahlbrand S, Patterson A, Pourmand N. Detection of point mutations associated with antibiotic resistance in Pseudomonas aeruginosa. International journal of antimicrobial agents. 1397;34(5):414-8.(In Persian)Guerra-Santos LH, Kappeli O, Fiechter A. Dependence of Pseudomonas aeruginosa continous culture biosurfactant production on nutritional and environmental factors. Applied Microbiology and Biotechnology. 1986;24(6):443-8.Guoyan Wu et al, Evaluation of agar dilution and broth microdilution methods to determine the disinfectant susceptibility, The Journal of Antibiotics (2015) 68, 661–665; doi:10.1038/ja.2015.51