Experimental study of the effect of Autologous Platelet Supernatant on the healing of corneal born ulcer in rabbit

Document Type : Original Article

Authors

1 Semnan

2 Graduate, , Faculty of Veterinary Medicine,, Islamic Azad University, Garmsar branch

10.22075/jvlr.2024.32530.1078

Abstract

A corneal ulcer is one of the most common eye diseases, which can be caused by trauma and chemical or some microbial agents. This study was carried out to investigate the effects of autologous platelet supernatant (PS) on corneal burn ulcer healing in 30 adult male New Zealand white rabbits. After general anesthesia, alkali wounds were created on the right corneas of rabbits by applying a 6 mm round filter paper, soaked in 1 M NaOH for 60 s. All rabbits were divided into three groups (n=10). In group A, platelet supernatant and in group B, atropine plus ciprofloxacin (AC) eye drops were used (one drop/12 h for 10 days). In group C, rabbits received normal saline, as the control group. Rabbit corneas were collected for histological examination on days 5 and 10. Histopathological factors such as epithelialization, vacuolar changes in epithelium and inflammation were evaluated. According to histopathological findings, a significant difference was observed between the PS group and with two another groups, while no significant difference was found between the AC group and the control group. The results from this study demonstrate that platelet supernatant can significantly promote the healing process of corneal alkali burn in rabbits.
 

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Journal of Veterinary Laboratory Research
Volume 14, Issue 2 - Serial Number 22
February 2023
Pages 121-129

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