Haematological changes and molecular detection of Anaplasma phagocytophilum in tick infested dogs in Isfahan Provice,Iran.

Document Type : Original Article

Authors

1 Department of clinical science,Veterinary faculty ,Shahrekord Branch,,Islamic Azad University,,Shahrekord,Iran

2 Department of clinical scince, veterinary faculty,Shrekord Branch,Islamic Azad university,Shahrekord, Iran

3 Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abstract

In this study, 100 blood samples of tick-infested dogs in Isfahan were collected and examined for molecular detection by Nested polymerase chain reaction (Nested PCR) method. Seven blood samples were found to be infected with Anaplasma phagocytophilum and their results were positive. Hematological studies of positive samples were showed that, in one of them, severe anemia, low hematocrit and neutrophillia without leukocytosis was seen.In four of them, anemia and neutrophilia (in 2 of them had leukocytosis also) was seen. and in two other positive samples, only neutrophilia was seen. In morbidity of the Anaplasma phagocytophilum infection in tick infested dogs, our examination and stastical analysis of data reveals that, age and contamination of the environement were affected but the genus were not affected the incidense of the disease.This study were conducted in four seasons of one year and it was done by the first time in Esfahan,middle province of Iran.
 

Keywords

Main Subjects


نعمان. م،1396، " مروری بر آناپلاسموزیس و وضعیت شیوع آناپلاسما مارجیناله در گاوهای ایران و جهان،" فصلنامه دامپزشکی 3(30).
 
Cardoso, L., Mendão, C., Luís Madeira de Carvalho.2012. Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and Leishmania infantum in apparently healthy and CVBD-suspect dogs in Portugal-a national serological study. Parasites & Vectors. 5(1), p.62.
 
M.J., Chandrashekar, R., Eberts, M.D., Cyr, K.E., Diniz, P.P., Mainville, C., Hegarty,B.C., Crawford, J.M., Breitschwerdt, E.B..2008. Serological and molecular prevalence of Borrelia burgdorferi, Anaplasma phagocytophilum and Ehrlichia species in dogs from Minnesota.Vector Borne Zoonotic Diseases 8,pp.455–464
 
Costa-Júnior, L.M., Rembeck,K. L.M.F. PassosM.F.B. Ribeiro.2013.“Factors associated with epidemiology of Anaplasma platys in dogs in rural and urban areas of Minas Gerais State, Brazil. Preventive veterinary medicine. 109(3-4), pp.321-326.
 
Dyachenko V., Geiger C.  N.PantchevM. Majzoub  L. Bell-Sakyi , , R. k Straubinger.2013. Isolation of canine Anaplasma phagocytophilum strains from clinical blood samples using the Ixodes ricinus cell line IRE/CTVM20. Veterinary microbiology. 162(2-4),pp.980-986.
 
Greene E.Craig, Jane Sykes. 2012. Infectious diseases of the dogs and cat,Elsivier. 4th edition. 1,244-259.
 
Hamidinejad H., Bahrami S., Mosalanejad B., Pahlavan S.. 2018. First molecular survey on Anaplasma phagocytophilum revealed high prevalence in rural dogs from Khuzestan province,Iran. Iran J Parasitol. 14, No.2,pp. 297-302.
 
Jensen J, Simon D, Murua Escobar H, Soller JT, Bullerdiek J..2007. Anaplasma phagocytophilum in dogs in Germany. Zoonoses Public Health. 54(3-4),p.169.
 
Josephus J. Fourie, Alec Evans, Michel LabuschagneD. Crafford, M.Madder, M. Pollmeier, B. Schunack.  2019. Transmission of Anaplasma phagocytophilum by Ixodes ricinus ticks feeding on dogs and artificial membranes. Parasites & Vectors. 12,p.136.
 
Kim JH, Lee CS, Moon C, Kwak YG, Kim BN, Kim ES, Kang JM, Park WB, Park SW..2006. Co-Infection of scrub typhus and human granulocytic Anaplasmosis in Korea. J Korean Med Sci.14;34(39),pp. 257.
 
Krämer, F.,Roland Schaper,B.Schunack,A.Połozowski.,J. Piekarska, 2014. Serological detection of Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato and Ehrlichia canis antibodies and Dirofilaria immitis antigen in a countrywide survey in dogs in Poland. Parasitology research. 113(9), pp.3229-3239.
 
Michael D Parkins,Deirdre L Church, Xiu Yan Jiang,D.B.G.. 2009. Human granulocytic Anaplasmosis-First reported case in Canada,Can J Infect Dis Med Microbiol, 20(3),pp.100-102.
 
Moroff, S., I. Sokolchik, T.W., C.W.. 2014. Detection of antibodies against Anaplasma phagocytophilum in dogs using an automated fluorescence based system. The Veterinary Journal. 202(2), pp.348-352.
 
Vincenzo Veneziano, D.P, B.N., F.DP.. 2017. Seroprevalence and risk factors associated with E.canis,Anaplasma spp,Borrelia burgdorfi senso lato and Dirofilaria immitis in hunting dogs from southern Itlay. Parasitology research.116,pp. 2651-2660.
 
Yabsley, M.J., McKibben, J., M.CN,C.PF.,. 2008. Prevalence of Ehrlichia canis, Anaplasma platys, Babesia Canis Vogeli, Hepatozoon Canis, Bartonella Vinsonii Berkhoffii, and Rickettsia spp. in dogs from Grenada. Veterinary parasitology. 151(2-4), pp.279-285.
 
YousefiA., M. R. Chaechi Nosrati,A. Golmohammadi, S. A. .2019.Molecular detection of Anaplasma phagocytophilum as a zoonotic agent im owned and straydogs in Tehran,Iran., Archives of Razi Institute. Archive 4, volume74,pp33-38.