Design and Usage of Semi-Nested PCR Technique for Detection of Theielria annulata and Theileria orientalis in Cattle

Abstract

AbstractTropical Theileriosis, due to Theileria annulata, is one of the major diseases of cattle in some tropical and sub-tropical countries that give rise to some serious economic losses in livestock industries in the involved countries such as Iran, every year. So far, Theileria annulata was identified by morphological, serological and molecular procedures in cattle of Iran. However, Theileria orientalis, which seems to be non-pathogenic, was only detected by morphological and serological methods in the cattle of Iran. Therefore, in the present investigation, for the first time in Iran we were able to differentiate two genus of Theielria and Babesia and also two species of Theielria annulata and Theileria orientalis by PCR and semi-nested PCR technique with designing primers of Bba-S, Bba-A, Tbs-S, Tbs-A, To-S and Ta-S.

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