Determination of Polymorphism of FecB gene in Najdi and native goats of Khouzestan province by PCR-RFLP

AbstractIran is one of the importanct centers for breeding sheep and goats.Najdi and native goats in Khouzestan province are one of those Iranian goats which have litter size and their average litter sizes are 46 and 26 percent respectively. It is reported that Booroola gene has additive for litter size and ovulation rate in goats. In this study 100 blood samples were collected of Najdi (50 goat) and Native goats (50 goats). DNA of blood samples was extracted by modified salting out method. The DNA was evaluated by spectrophotometer and agarose gel. Site of mutation was amplified using specific primers and PCR product (190 bp bands) was determined by agarose gel electrophoresis, and then the products PCR were digested with AvaII enzyme. The homozygous carrier goats with FecB mutation (BB) have a 160 bp and 30 bp bands, the noncarriers (++) have a 190 bp bands, whereas heterozygotes (B+) have 160, 30 and 190 bp bands. Results ِdid not show any polymorphism for FecB gene in khouzestanian goats. All of goats were wild type (++) so mutation of FecB gene is not cause of prolificacy in khouzestanian goats. Further research is required to evaluate fecundity gene and genotyping of khouzestanian goats.