The study of periplasmic and outer membrane proteins patterns in Salmonella enterica subsp. enterica serovar Abortusovis strains isolated from Iran

Abstract

  Abstract:  Aim of this study was comparison of periplasmic and outer membrane proteins (OMPs) patterns between Salmonella abortus ovis strains isolated from Iran. Outer membrane proteins were prepared by sonication in triton X-100. Periplasmic proteins were extracted by 500 Mm Sucrose osmotic shock. Periplasmic and OMPs extracted proteins were analyzed by SDS-PAGE and silver staining. The results showed that there is considerable difference between periplasmic protein profiles of S. abortus ovis strains. The outer membrane proteins patterns were approximately similar among the strains. The protein profile differences and number of protein bands showed in figure and tables. In fact these variations indicating a certain genotypic distance between bacterial strains that isolated from different geographical area of Iran and may be useful for describing the epidemiology or at least genetic relatedness of Salmonella abortus ovis wild types.    

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