Histopathological evaluation of topical autologous platelet-rich fibrin in femoral head and neck ostectomy in rabbits

Document Type : Original Article

Authors

1 Graduated from faculty of veterinary medicine, semnan university, semnan, Iran

2 Semnan

3 Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University Semnan. Iran

4 Department of clinical sciences, faculty of veterinary medicine, semnan university, semnan. Iran.

Abstract

This study evaluated the histopathological changes following topical administration of PRF in femoral head and neck ostectomy (FHO) in rabbits. 20 New Zealand white rabbits underwent FHO. In the treated group, surgical site filled with PRF. After two and four weeks, all animals were euthanised and femur with the repair tissues around it was cut approximately 2 cm far from the head of femur. For microscopic study, some slides were prepared from samples through serial sectioning and were stained with H&E and Mason Trichrome. Qualitative changes such as granulation tissues, cartilage and bone formation were observed and noted. The results of this study shows that in PRF treated group, ratio of cartilage tissue formation to granulation tissue as well as formation of bone to granulation tissue is significantly more than these parameters in control group. This result indicates to faster healing on the damaged area in treated group. Also in this Group cartilage and bone tissues formation in the healing process was more orderly. The results indicate that the use of PRF has been able to accelerate healing of bone tissue that is cut at the head of the rabbit’s femur. So it seems that the use of PRF as a treatment protocol to accelerate the healing period of bone tissue in these cases could be suggested.

Keywords

References

Bansal, S., Garg, A., Khurana, R., Chhabra, P. 2017. Platelet-rich fibrin or platelet-rich plasma – which one is better? an opinion. Indian Journal of Dental Sciences. 9(5), 49-52.
Barbeck, M., Najman, S., Stojanovic, S., Mitic, Z., Zivkovic, J.M., Choukroun, J., Predrag, K., Sader, R., Kirkpatrick, C.J., Ghanaati, S. 2015. Addition of blood to a phycogenic bone substitute leads to increased in vivo vascularization. Biomedical Materials. 10(5), 1-15.
Borie, E., Olivi, D.G., Orsi, I.A., Garlet, K., Weber, B., Beltran, V. 2015. Platelet-rich fibrin application in dentistry: a literature review. International Journal of Clinical Experimental Medicine. 15(5), 7922-7929.
Chang, I.C., Tsai, C.H., Chang, Y.C. 2010. Platelet-rich fibrin modulates the expression of extracellular signal-regulated protein kinase and osteoprotegerin in human osteoblasts. Journal of Biomedical Materials Research Part A. 95(1), 327–332.
Chang, Y.C., Zhao, J.H. 2011. Effects of platelet-rich fibrin on human periodontal ligament fibroblasts and application for periodontal infrabony defects. Australian Dental Journal. 56, 365–371.
Choukroun, J., Diss, A., Simonpieri, A., Girard, M.O., Schoeffler, C., Dohan, S.L., Dohan, A.J.J., Mouhyi, J., Dohan, D.M. 2006. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surgury, Oral Medecine, Oral Patholpgy, Oral Radiology and Endodontology. 101(3), 56-60.
DeCamp, C.E., Johnston, S.A., Dejardin, L.M., Schaefer, S.L. 2016. Brinker, Piermattei and Flo's Handbook of Small Animal Orthopedics and Fracture Repair. 5th ed. Elseviers Publishing, USA. 508-515.
Diss, A., Dohan, D.M., Mouhyi, J., Mahler, P. 2008. Osteotome sinus floor elevation using Choukroun's platelet-rich fibrin as grafting material: a 1-year prospective pilot study with microthreaded implants. Oral Surgury, Oral Medecine, Oral Pathology and Oral Radiology. 105(5), 572-579.
Dohan Ehrenfest, D.M., Del Corso, M. Diss, A., Mouhyi, J., Charrier, J.B. 2010. Three-dimensional architecture and cell composition of a Choukroun’s platelet-rich fibrin clot and membrane. Journal of Periodontology. 81(4), 546-555.
Griffin, X.L. Wallace, D., Parsons, N., Costa, M.L. 2012. Platelet rich therapies for long bone healing in adults (Review). Cochrane Database of Systematic Reviews. 11(7), 1-21.
Guo, S., Dipietro, L.A. 2010. Factors affecting wound healing. Journal of Dental Research. 89(3), 219–229.
Joseph, V.R., Sam, G., Amol, N.V. 2014. Clinical evaluation of autologous platelet rich fibrin in horizontal alveolar bony defects. Journal of Clinical and Diagnostic Research. 8(11), 43–47.
Kim, T.H., Kim, S.H., Sandor, G.K., Kim, Y.D. 2014. Comparison of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) in rabbit-skull defect healing. Archives of Oral Biology. 59(5), 550-558.
Kiran, N.K., Mukunda, K.S., Tilak Raj, T.N. 2011. Platelet concentrates: A promising innovation in dentistry. International Journal of Dental Sciences and Research. 2, 50–61.
Kumar, K.R., Genmorgan, K., Abdul Rahman, S.M., Rajan, M.A., Kumar, T.A., Prasad, V.S. 2016. Role of plasma-rich fibrin in oral surgery. Journal of Pharmacy and Bioallied Sciences. 8(1), 36-38.
Martin, P., Leibovich, S.J. 2005. Inflammatory cells during wound repair: the good, the bad and the ugly. Trends in Cell Biology. 15(11), 599-607.
Pradeep, A.R., Rao, N.S., Agarwal, E., Bajaj, P., Kumari, M., Naik, S.B. 2012. Comparative evaluation of autologous platelet-rich fibrin and platelet-rich plasma in the treatment of 3-wall intrabony defects in chronic periodontitis: a randomized controlled clinical trial. Journal of Periodontology. 83(12), 1499-1507.
Sharma, A., Pradeep, A.R. 2011. Autologous platelet-rich fibrin in the treatment of mandibular degree II furcation defects: a randomized clinical trial. Journal of Periodontology. 82(10), 1396-1403.
Sunitha Raja, V., Munirathnam Naidu, E. 2008. Platelet-rich fibrin: evolution of a second-generation platelet concentrate. Indian Journal of Dental Research. 19(1), 42-46.
Thor, A., Hong, J., Zelin, G., Sennerby, L. Rasmusson, L. 2017. Correlation of platelet growth factor release in jawbone defect repair–astudy in the dog mandible. The International Journal of Oral Maxillofacial Implants. 15(5), 759-768.
 

Files

Share

How to cite

Statistics