Histopathological evaluation of topical autologous platelet-rich fibrin in femoral head and neck ostectomy in rabbits

Document Type : Original Article

Authors

1 Graduated from faculty of veterinary medicine, semnan university, semnan, Iran

2 Semnan

3 Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University Semnan. Iran

4 Department of clinical sciences, faculty of veterinary medicine, semnan university, semnan. Iran.

Abstract

This study evaluated the histopathological changes following topical administration of PRF in femoral head and neck ostectomy (FHO) in rabbits. 20 New Zealand white rabbits underwent FHO. In the treated group, surgical site filled with PRF. After two and four weeks, all animals were euthanised and femur with the repair tissues around it was cut approximately 2 cm far from the head of femur. For microscopic study, some slides were prepared from samples through serial sectioning and were stained with H&E and Mason Trichrome. Qualitative changes such as granulation tissues, cartilage and bone formation were observed and noted. The results of this study shows that in PRF treated group, ratio of cartilage tissue formation to granulation tissue as well as formation of bone to granulation tissue is significantly more than these parameters in control group. This result indicates to faster healing on the damaged area in treated group. Also in this Group cartilage and bone tissues formation in the healing process was more orderly. The results indicate that the use of PRF has been able to accelerate healing of bone tissue that is cut at the head of the rabbit’s femur. So it seems that the use of PRF as a treatment protocol to accelerate the healing period of bone tissue in these cases could be suggested.

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