Study on Salmonella spp. in broiler farms around Ghaemshahr: Determination of serotypes and their drugs resistance

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Abstract
Salmonella spp. is Gram-negative bacteria and belongs to entrobacteriaceae family. Salmonella spp. is one of the most important zoonotic agents with worldwide distribution. The aims of this study were to isolate Salmonella from poultry farms around Ghaemshahr city, identify the serotypes, and determine the drug resistance patterns of the isolated Salmonella spp. A total number of 2280 samples were randomly collected from freshly dropped feces of broiler chickens in different flocks. Each 10 samples were pooled and processed for Salmonella isolation according to the standard procedures. Serotyping was performed by slide agglutination test to determine the O and H antigens of the isolates. The antigens included A to D polyvalent, and O2, O4, O5, O9, O12, H2, H6, HL, Hgm monovalent antisera. Antimicrobial susceptibility of the isolates against 30 antimicrobial agents was determined by using standard disk diffusion method. Out of 2280 samples (228 pooled-samples), 30 Salmonella isolates were recovered. Serotyping revealed that two isolates were Salmonella spp. enteritidis and 28 isolates belonged to serogroup C. All 30 Salmonella isolates were susceptible to levofloxacin and Fosbac. Also, the highest resistances were related to streptomycin, furazolidone, nalidixic acid, tetracycline, and lincospectin. Multi-resistance to drugs was common among the Salmonella isolates and ranged between 2 to 17. There were 29 resistance patterns. The results of this study showed the presence of Salmonella spp. infection among broiler chickens in Ghaemshar regions and the occurrence of antimicrobials resistance among the isolates. These findings are important for Iranian poultry industry and of concern to public health. 

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Journal of Veterinary Laboratory Research
Volume 5, Issue 2 - Serial Number 10
November 2013
Pages 85-94

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