Detection and Quantification of Oxytetracycline Residues in Raw Semnan Milk Using High-Performance Liquid Chromatography (HPLC) Method

Document Type : Original Article

Authors

1 Faculty of Veterinary Medicine, Semnan, Semnan, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

3 Department of Food Hygiene, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

4 , Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

10.22075/jvlr.2025.38791.1183

Abstract

Tetracyclines are a widely used, broad-spectrum class of antibiotics prescribed to manage mastitis and various diseases in dairy herds.  The improper and excessive use of antibiotics, along with failure to observe withdrawal periods, has led to the presence of drug residues in food products of animal origin. The present study aimed to determine the level of oxytetracycline in raw milk consumed in the city of Semnan. This cross-sectional study was conducted during the autumn. Thirty-six samples were collected from a milk platform in the city of Semnan. The samples were then analyzed for oxytetracycline residues using High-Performance Liquid Chromatography (HPLC). The concentration of oxytetracycline isolated in all samples was less than the Maximum Residue Limit (MRL) of 0.1 µg/mL, with the exception of one sample that was above the limit. Given the presence of drug residues in milk found in this study and others, further investigations into the health of products from treated animals for human consumption should be a priority. The prudent use of antimicrobial drugs and adherence to their withdrawal periods can prevent the adverse effects and harm associated with them.

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References

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Journal of Veterinary Laboratory Research
Volume 17, Issue 2 - Serial Number 28
in processing
October 2025
Pages 161-169

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