Comparative Detection of Salmonella typhimurium from Imported Frozen Chickens using Traditional Culture Method and Real Time PCR

Articles in Press

Document Type : Original Article

Authors

1 Food Hygiene Department, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

10.22075/jvlr.2025.37919.1172

Abstract

Chicken meat is recognized as a cost-effective, healthy source of high-quality protein with low fat content. One of the primary causes of foodborne illnesses is Salmonella and its various serotypes. Salmonella typhimurium is a major public health issue worldwide. This study evaluates the comparative separation and diagnosis of Salmonella spp. and Salmonella typhimurium in 73 imported frozen chicken samples by culture and simultaneous Real Time PCR with specific primers. The results indicated that, out of the 73 frozen chicken samples analyzed through microbial cultivation for Salmonella detection, 5 samples were identified as Salmonella spp. (6.8%). Among these, 2 samples were specifically diagnosed as Salmonella typhimurium using serological method (2.7%). In molecular method, 9 samples were identified as positive (12.3%), with 4 of these PCR-positive samples confirmed as Salmonella typhimurium (5.4%). Thus, the comparative results between the two testing methods revealed that the Real-Time PCR method was 1.8 times (approximately double) more effective than the conventional cultivation method. In conclusion, the monitoring of frozen imported chickens for Salmonella contamination demonstrated that Real-Time PCR offers significant advantages in terms of accuracy and speed compared to traditional cultivation methods.

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Journal of Veterinary Laboratory Research

Articles in Press, Accepted Manuscript
Available Online from 25 May 2026

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