Semnan UniversityJournal of Veterinary Laboratory Research2821-05066120140522Cover of Journal of Veterinary Laboratory ResearchCover of Journal of Veterinary Laboratory Research124910.22075/jvlr.2017.1249FAJournal Article20170128https://jvlr.semnan.ac.ir/article_1249_2ba8698b79439589fdd2b0f7218d8b07.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-05066120140522ContentContent125010.22075/jvlr.2017.1250FAJournal Article20170128https://jvlr.semnan.ac.ir/article_1250_81e5f81db77c596492e6f1a5a792ed53.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-0506612014052213125110.22075/jvlr.2017.1251FAJournal Article20170128https://jvlr.semnan.ac.ir/article_1251_838e8afb1ca34354ac209f53d90c3a43.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-0506612014052259125210.22075/jvlr.2017.1252FAAliEslamiJournal Article20170128https://jvlr.semnan.ac.ir/article_1252_a284df1155ec3e67286080500df36a9a.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-05066120140522Study on the helminth infections of digestive tracts in stray dogs from Qom Province, IranStudy on the helminth infections of digestive tracts in stray dogs from Qom Province, Iran1118125310.22075/jvlr.2017.1253FAGorbani, M, N.Eslami, A.Abdollrahmani, SH.Youssefi, A.Journal Article20170128Abstract
From March 2010 to May 2011, 61 stray dogs were captured from Qom province and euthanized then aupospied for digestive tracts helminth infections. Cestodes and acanthcethala were relaxed in tap water, stained by alum acetocarmine and cleared in xylol and nematodes cleared in lactophenol for identification. In the present study, 86.88% of dogs were infected with cestodes, 57.37% with nematodes and 3.27% with acanthocephalanes. Helminthes fauna and their prevalence were as follows: Taenia hydagena (32.79%), Echinococcus granulosus (26.22%), Dipylidium caninum (49%), Taenia mulpiceps (14. 75%), and Mecestides lineatus (1.36%), Toxocara canis (37.70%), Toxascaris leonina (21.31%), Rictularia cahirencis (3.27%) and Macracanthorhynchus hirudinaceus (4.91%). The spectrum of intensity of all worms was 2-24, except E. granulosus that was being between 700-3000. All helminths are transmissible to carnivores, and those T. hydagena, E. granulosus, T. multiceps to ruminants and those of E. granulosus, D. canicum, T. multiceps and T. canis to human beings.Abstract
From March 2010 to May 2011, 61 stray dogs were captured from Qom province and euthanized then aupospied for digestive tracts helminth infections. Cestodes and acanthcethala were relaxed in tap water, stained by alum acetocarmine and cleared in xylol and nematodes cleared in lactophenol for identification. In the present study, 86.88% of dogs were infected with cestodes, 57.37% with nematodes and 3.27% with acanthocephalanes. Helminthes fauna and their prevalence were as follows: Taenia hydagena (32.79%), Echinococcus granulosus (26.22%), Dipylidium caninum (49%), Taenia mulpiceps (14. 75%), and Mecestides lineatus (1.36%), Toxocara canis (37.70%), Toxascaris leonina (21.31%), Rictularia cahirencis (3.27%) and Macracanthorhynchus hirudinaceus (4.91%). The spectrum of intensity of all worms was 2-24, except E. granulosus that was being between 700-3000. All helminths are transmissible to carnivores, and those T. hydagena, E. granulosus, T. multiceps to ruminants and those of E. granulosus, D. canicum, T. multiceps and T. canis to human beings.https://jvlr.semnan.ac.ir/article_1253_b495ce63ede0f4efc9eec62cb947c162.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-05066120140522The influence of various nutritional materials on maturation of Eisenia foetida in vermicompost industryThe influence of various nutritional materials on maturation of Eisenia foetida in vermicompost industry1926125410.22075/jvlr.2017.1254FAJahani , Z.Meshgi , B.Journal Article20170128Abstract
Vermicomposting could be a suitable technology for the transformation of different waste into available products. Vermicompost industry is the based on earthworm. Although, there are more than 7000 species of earthworm in the word, but Eisenia foetida is one of the most important for soil fertility. Of course, several studies showed which earthworms the variety of activities such as healthy food as well as in medicine. The aim of this study was to assess, the influence of different waste such as vegetable, paper, banana, carrot, bread wastes on the growth and development of E. foetida. The effect of various wastes on growth, reproduction and adult's weight of an earthworm E. foetida was studied under identical laboratory conditions. In the use of banana peel, flour and paper filings baby number from 500 has risen in 2000, 1800 and 1500 respectively. The maximum amount of vermicomposted soil compared with controls, were related to the paper filings and lettuce (145 and 106 g respectively). Generally the earthworm population, weight of adults and the amount of soil vermicompost, showed that paper filings and banana peel were the best medium for earthworm E. foetida.Abstract
Vermicomposting could be a suitable technology for the transformation of different waste into available products. Vermicompost industry is the based on earthworm. Although, there are more than 7000 species of earthworm in the word, but Eisenia foetida is one of the most important for soil fertility. Of course, several studies showed which earthworms the variety of activities such as healthy food as well as in medicine. The aim of this study was to assess, the influence of different waste such as vegetable, paper, banana, carrot, bread wastes on the growth and development of E. foetida. The effect of various wastes on growth, reproduction and adult's weight of an earthworm E. foetida was studied under identical laboratory conditions. In the use of banana peel, flour and paper filings baby number from 500 has risen in 2000, 1800 and 1500 respectively. The maximum amount of vermicomposted soil compared with controls, were related to the paper filings and lettuce (145 and 106 g respectively). Generally the earthworm population, weight of adults and the amount of soil vermicompost, showed that paper filings and banana peel were the best medium for earthworm E. foetida.https://jvlr.semnan.ac.ir/article_1254_884ce4bb65d328ecb03c598409e2b168.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-05066120140522Effects of Macrogard on growth, haematological parameters and resistance of rainbow trout (Oncorhynchus mykiss) Challenged with Streptococcus iniaeEffects of Macrogard on growth, haematological parameters and resistance of rainbow trout (Oncorhynchus mykiss) Challenged with Streptococcus iniae2736125510.22075/jvlr.2017.1255FAPourmozaffar, S.Nafisi Bahabadi, M.Mohajeri, J.Mohammady, M.Salimi Bejestani, M.R.Journal Article20170128Abstract
The purpose of this study was to evaluate the effects of orally administered β-glucan (Macrogard) extracted from Saccharomyces cerevisiae (Macrogard) 1 and 2 gr per kg of feed on growth factors and hematological parameters including hematocrit, hemoglobin, MCV, MCH, MCHC, total red and white blood cells of rainbow trout (28.20± 2.50 gr). The fish were divided into 3 groups including control and Macrogards treatments. At the end of the experimental period (45 days) Bloods were taken and bloods parameters were evaluated. The results showed that the fish growth and FCR in Macrogard treatments were significantly positive compared to control treatment (p< 0/05). Measurement of blood parameters such as hematocrit, hemoglobin, MCV, MCH, MCHC was not significantly different from control (p>0/05). A significant increase in the number of white blood cells in the treatments compared to the control was observed (p<0/05). The differential count of white blood cell, neutrophil count in Macrogards treatments showed significantly different with control (p<0/05). The results of this study showed that the oral administration of Macrogard enhances growth and improves the immune responses of fish, through the proliferation white blood cells particularly neutrophils population of rainbow trout and resistance to environmental stress and pathogens. Abstract
The purpose of this study was to evaluate the effects of orally administered β-glucan (Macrogard) extracted from Saccharomyces cerevisiae (Macrogard) 1 and 2 gr per kg of feed on growth factors and hematological parameters including hematocrit, hemoglobin, MCV, MCH, MCHC, total red and white blood cells of rainbow trout (28.20± 2.50 gr). The fish were divided into 3 groups including control and Macrogards treatments. At the end of the experimental period (45 days) Bloods were taken and bloods parameters were evaluated. The results showed that the fish growth and FCR in Macrogard treatments were significantly positive compared to control treatment (p< 0/05). Measurement of blood parameters such as hematocrit, hemoglobin, MCV, MCH, MCHC was not significantly different from control (p>0/05). A significant increase in the number of white blood cells in the treatments compared to the control was observed (p<0/05). The differential count of white blood cell, neutrophil count in Macrogards treatments showed significantly different with control (p<0/05). The results of this study showed that the oral administration of Macrogard enhances growth and improves the immune responses of fish, through the proliferation white blood cells particularly neutrophils population of rainbow trout and resistance to environmental stress and pathogens. https://jvlr.semnan.ac.ir/article_1255_3bf55bbad370a8fcad1d09b005e278c2.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-05066120140522Effect of Thistle seeds on hepatic gene expression of interleukin 6 (IL-6) in broiler chickens fed with Aflatoxin B1Effect of Thistle seeds on hepatic gene expression of interleukin 6 (IL-6) in broiler chickens fed with Aflatoxin B13744125610.22075/jvlr.2017.1256FAFrouzanmehr, M.Montazer Torbati, M.B.Sarir, H.Fani Makki, O.Mohammadi, H.R.Ebrahimzadeh, A.Journal Article20170128Abstract
The objective of the present study was to evaluate the efficacy of milk thistle seeds (MTS) to ameliorate gene expression of interleukin-6 (IL-6) in the livers of broiler chicks fed with aflatoxin B1 (AFB1). 96 one-day-old (Ross 308) broiler chicks were used. They were randomly assigned to four treatments, 4 replicates and 6 birds in each unit. Group (1) was kept as a control and other groups received feed containing MTS and AFB1 for 3 weeks, i.e. 2) basal diet supplemented with 500 ppb of AFB1 per kg of feed, 3) basal diet supplemented with MTS (0.5%) and 4) basal diet supplemented with MTS (0.5%) and 500 ppb of AFB1 per kg of feed. On day 35 livers were collected (3 per treatment) to evaluate changes in the expression of IL-6 gene after reverse transcription of RNA extracted from liver tissue were taken. In addition, polymerase chain reaction (PCR) amplification was performed for IL-6. The result revealed expression of IL-6 decreased in treatments which is received AF while MTS alone increased IL-6 expression. The variables that were evaluated in this study showed that MTS had significant efficacy in diminishing the aflatoxin negative effects on the chickens.
Abstract
The objective of the present study was to evaluate the efficacy of milk thistle seeds (MTS) to ameliorate gene expression of interleukin-6 (IL-6) in the livers of broiler chicks fed with aflatoxin B1 (AFB1). 96 one-day-old (Ross 308) broiler chicks were used. They were randomly assigned to four treatments, 4 replicates and 6 birds in each unit. Group (1) was kept as a control and other groups received feed containing MTS and AFB1 for 3 weeks, i.e. 2) basal diet supplemented with 500 ppb of AFB1 per kg of feed, 3) basal diet supplemented with MTS (0.5%) and 4) basal diet supplemented with MTS (0.5%) and 500 ppb of AFB1 per kg of feed. On day 35 livers were collected (3 per treatment) to evaluate changes in the expression of IL-6 gene after reverse transcription of RNA extracted from liver tissue were taken. In addition, polymerase chain reaction (PCR) amplification was performed for IL-6. The result revealed expression of IL-6 decreased in treatments which is received AF while MTS alone increased IL-6 expression. The variables that were evaluated in this study showed that MTS had significant efficacy in diminishing the aflatoxin negative effects on the chickens.
https://jvlr.semnan.ac.ir/article_1256_e3251075554389fe91d17a794861d47b.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-05066120140522The determination of prevalence of Neospora caninum in aborted fetuses in dairy cattle of Shahrekord area, Chahar Mahal Bakhtiari province, by Nested-PCRThe determination of prevalence of Neospora caninum in aborted fetuses in dairy cattle of Shahrekord area, Chahar Mahal Bakhtiari province, by Nested-PCR4550125710.22075/jvlr.2017.1257FARafati, N.Jaafarian,M.Journal Article20170128Abstract
Neospora caninum is one of the causes in aborted cattle in the world, accompanied with millions dollars economic loses. The aim of this study was to determine the prevalence of bovine Neospora caninum in aborted fetuses in shahrekord, by Nested-PCR method. Among the 100 aborted fetuses brains, 11 cases ( 11%) were infected. According to our results, Neospora caninum is one of important factors of abortion in dairy cows in Shahrekord.
Abstract
Neospora caninum is one of the causes in aborted cattle in the world, accompanied with millions dollars economic loses. The aim of this study was to determine the prevalence of bovine Neospora caninum in aborted fetuses in shahrekord, by Nested-PCR method. Among the 100 aborted fetuses brains, 11 cases ( 11%) were infected. According to our results, Neospora caninum is one of important factors of abortion in dairy cows in Shahrekord.
https://jvlr.semnan.ac.ir/article_1257_cd758e8f59dfdf06a852adad277986ca.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-05066120140522A molecular survey on the prevalent of Blastocystis infection in some cattle in Khorramabad city, IranA molecular survey on the prevalent of Blastocystis infection in some cattle in Khorramabad city, Iran5158125810.22075/jvlr.2017.1258FABadparva, E.Kheirandish, F.Sadraee , J.Forouzandeh, M.Journal Article20170128Abstract
Blastocystis is a zoonotic and anaerobic protozoan that transmitted directly by the cyst via contaminated water and food. It is cosmopolitan in distribution and its prevalence is higher in the community with poor hygiene. Stool samples (n=196) were collected from cattle of slaughterhouse (98 samples) and dairy farms (98 samples). DNA extracted for each sample and analyzed by PCR for Blastocystis contamination. Out of 196 samples , 19 ( 9.6% ) samples were positive for Blastocystis that 12 ( 12.2% ) samples were form cattle of slaughterhouse and 7 ( 7.1% ) samples from cattle of dairy farms. Since cattle are as an important source of food for the supply of meat and other products in Iran, hence investigation of the contamination and possibility of transmission to humans is of particular importance.
Abstract
Blastocystis is a zoonotic and anaerobic protozoan that transmitted directly by the cyst via contaminated water and food. It is cosmopolitan in distribution and its prevalence is higher in the community with poor hygiene. Stool samples (n=196) were collected from cattle of slaughterhouse (98 samples) and dairy farms (98 samples). DNA extracted for each sample and analyzed by PCR for Blastocystis contamination. Out of 196 samples , 19 ( 9.6% ) samples were positive for Blastocystis that 12 ( 12.2% ) samples were form cattle of slaughterhouse and 7 ( 7.1% ) samples from cattle of dairy farms. Since cattle are as an important source of food for the supply of meat and other products in Iran, hence investigation of the contamination and possibility of transmission to humans is of particular importance.
https://jvlr.semnan.ac.ir/article_1258_26588e932c7ccfa1df309280702fe1b5.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-05066120140522Assesement of the effects of seasons and some hematological and biochemical parameters in subclinical mastitis ewes in province of Semnan, IranAssesement of the effects of seasons and some hematological and biochemical parameters in subclinical mastitis ewes in province of Semnan, Iran5966125910.22075/jvlr.2017.1259FAAhmadi-Hamedani, M.Narenji Sani, R.Journal Article20170128Abstract
This study was conducted to investigate the simultaneous effects of season and subclinical mastitis (SCM) on some hematological and biochemical parameters in ewes. Therefore 150 milk and blood samples were collected from SCM ewes and 992 milk and blood samples were collected from healthy ewes in spring and summer. 16.94% and 10.97% samples were positive for bacterial culture in spring and summer, respectively. There is not any significant difference in results of RBC and PCV parameters in ewes with SCM and healthy ewes in spring and summer but total protein concentrations and plasma fibrinogen in both groups of ewes were significantly higher in spring than summer (PË0.05). The results of the present study indicated that season and SCM has prominent effect on hematological and serum biochemical parameters in ewes.Abstract
This study was conducted to investigate the simultaneous effects of season and subclinical mastitis (SCM) on some hematological and biochemical parameters in ewes. Therefore 150 milk and blood samples were collected from SCM ewes and 992 milk and blood samples were collected from healthy ewes in spring and summer. 16.94% and 10.97% samples were positive for bacterial culture in spring and summer, respectively. There is not any significant difference in results of RBC and PCV parameters in ewes with SCM and healthy ewes in spring and summer but total protein concentrations and plasma fibrinogen in both groups of ewes were significantly higher in spring than summer (PË0.05). The results of the present study indicated that season and SCM has prominent effect on hematological and serum biochemical parameters in ewes.https://jvlr.semnan.ac.ir/article_1259_68a83eeb494a308fe5295da69428a507.pdfSemnan UniversityJournal of Veterinary Laboratory Research2821-05066120140522Investigation of veterinary attitudes towards perioperative pain management in Iranian Veterinary SchoolsInvestigation of veterinary attitudes towards perioperative pain management in Iranian Veterinary Schools6778126010.22075/jvlr.2017.1260FAVesal, N.Imani, H.Journal Article20170128Abstract
The prevention and alleviation of pain associated with surgery is the main responsibility of veterinarians. At present, no information regarding veterinary attitudes towards the management of pain in surgical patients in Iranian Veterinary Schools is available. In this study, the attitudes and the current practice of anesthesia and the availability and prescription of analgesic drugs for perioperative pain management in small and large animals have been investigated in 9 Iranian Veterinary Schools. In general, the results of this pilot study indicate that the management of pain in animals is suboptimal and inadequate. Although all clinics had access to several NSAIDs, actual prescription rate was low. Opioids were routinely available only in one clinic. In an attempt to improve the attitudes of veterinarians towards the management of pain, organizing continuing education programs on the topic of pain management and revision in the veterinary medical teaching curriculum are required. Availability of opioids may also play a significant role in the improvement of postoperative pain management in animals.Abstract
The prevention and alleviation of pain associated with surgery is the main responsibility of veterinarians. At present, no information regarding veterinary attitudes towards the management of pain in surgical patients in Iranian Veterinary Schools is available. In this study, the attitudes and the current practice of anesthesia and the availability and prescription of analgesic drugs for perioperative pain management in small and large animals have been investigated in 9 Iranian Veterinary Schools. In general, the results of this pilot study indicate that the management of pain in animals is suboptimal and inadequate. Although all clinics had access to several NSAIDs, actual prescription rate was low. Opioids were routinely available only in one clinic. In an attempt to improve the attitudes of veterinarians towards the management of pain, organizing continuing education programs on the topic of pain management and revision in the veterinary medical teaching curriculum are required. Availability of opioids may also play a significant role in the improvement of postoperative pain management in animals.https://jvlr.semnan.ac.ir/article_1260_f4573fc71c731d5c362f0d7860945b88.pdf