Semnan University Journal of Veterinary Laboratory Research 2821-0506 18 1 2026 03 21 Molecular detection of Coxiella burnetii among camel`s blood in Semnan County Molecular detection of Coxiella burnetii among camel`s blood in Semnan County 89 95 10654 10.22075/jvlr.2026.39562.1192 FA Omid Mohammadi Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran Hamidreza Mohammadi Department of Clinical science, Semnan University, Semnan, Iran 0000-0002-1592-9942 Mehran Ghaemi Bafghi Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University, Mashhad, Iran 0000-0002-4606-8161 Ali Mahdavi Department of Clinical sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran 0000-0002-5867-9293 Journal Article 2025 11 05 Q fever is an important zoonotic disease caused by <em>Coxiella burnetii</em>, which can be present in animals-especially camels-without clinical signs and act as a potential reservoir for human infection. This study aimed to molecularly detect and determine the prevalence of <em>C. burnetii</em> in camels from Semnan County using PCR. Blood samples were randomly collected from 50 camels, and data including age and sex were recorded. Samples were collected into EDTA tubes, transported on ice to the laboratory, and buffy coats were separated. DNA was extracted using a commercial kit according to the manufacturer’s instructions. PCR was performed using <em>C. burnetii</em>-specific primers with positive and negative controls, and amplified products were analyzed by agarose gel electrophoresis. The results showed that none of the samples exhibited the specific <em>C. burnetii</em> band. Accordingly, no evidence of Q fever infection was observed in the blood samples of camels from Semnan County. Considering the importance of this zoonotic disease, further studies on different livestock species across various regions of the country are recommended. Q fever is an important zoonotic disease caused by <em>Coxiella burnetii</em>, which can be present in animals-especially camels-without clinical signs and act as a potential reservoir for human infection. This study aimed to molecularly detect and determine the prevalence of <em>C. burnetii</em> in camels from Semnan County using PCR. Blood samples were randomly collected from 50 camels, and data including age and sex were recorded. Samples were collected into EDTA tubes, transported on ice to the laboratory, and buffy coats were separated. DNA was extracted using a commercial kit according to the manufacturer’s instructions. PCR was performed using <em>C. burnetii</em>-specific primers with positive and negative controls, and amplified products were analyzed by agarose gel electrophoresis. The results showed that none of the samples exhibited the specific <em>C. burnetii</em> band. Accordingly, no evidence of Q fever infection was observed in the blood samples of camels from Semnan County. Considering the importance of this zoonotic disease, further studies on different livestock species across various regions of the country are recommended. https://jvlr.semnan.ac.ir/article_10654_c370416bd42d48c75ceeba04434460f2.pdf