Semnan University Journal of Veterinary Laboratory Research 2821-0506 17 2 2026 03 16 Measurement of TSH, T3 and T4 in Darehshori, Iranian pure Arab and foreign blood Arab horses Measurement of TSH, T3 and T4 in Darehshori, Iranian pure Arab and foreign blood Arab horses 195 200 10537 10.22075/jvlr.2025.36050.1141 FA Ali Asghar Mozaffari Department of Clinical Studies, Veterinary School, Shahid Bahonar university of Kerman, iran 0000-0001-9468-6944 Mehrdad Shamsadini Research, Education and Extension Organization (AREEO), Razi Vaccine& Serum Research institute, Kerman Branch, Kerman, Iran Hesam Rafizadeh Shahi Doctor of Veterinary Medicine, Kerman, Iran Hossein Salajegheh Student of Veterinary Medicine, Shahid Bahonar University of Kerman, Iran. Journal Article 2024 11 26 The thyroid gland, a critical endocrine organ, regulates physiological functions throughout the body via the secretion of the hormones triiodothyronine (T3) and thyroxine (T4). Deviations in thyroid hormone levels can impact multiple organ systems and lead to diverse clinical manifestations. This study aimed to measure serum concentrations of thyroid-stimulating hormone (TSH), T3, and T4 in three equine breeds: Darehshori, Iranian Purebred Arab, and Foreign Blood Arab horses. A total of 120 blood samples (40 per breed) were collected from horse farms in Yazd, Iran. Following centrifugation, serum was separated and stored in freezer until analysis. Hormone concentrations were determined using commercial kits for T3 (Pishtaz Teb), T4 (monobound kit, USA), and TSH (ELISA, Saman Tajghiz Noor) according to manufacturers' protocols. The results demonstrated significant differences in TSH, T3, and T4 levels between Iranian Purebred Arab and Foreign Blood Arab horses. The thyroid gland, a critical endocrine organ, regulates physiological functions throughout the body via the secretion of the hormones triiodothyronine (T3) and thyroxine (T4). Deviations in thyroid hormone levels can impact multiple organ systems and lead to diverse clinical manifestations. This study aimed to measure serum concentrations of thyroid-stimulating hormone (TSH), T3, and T4 in three equine breeds: Darehshori, Iranian Purebred Arab, and Foreign Blood Arab horses. A total of 120 blood samples (40 per breed) were collected from horse farms in Yazd, Iran. Following centrifugation, serum was separated and stored in freezer until analysis. Hormone concentrations were determined using commercial kits for T3 (Pishtaz Teb), T4 (monobound kit, USA), and TSH (ELISA, Saman Tajghiz Noor) according to manufacturers' protocols. The results demonstrated significant differences in TSH, T3, and T4 levels between Iranian Purebred Arab and Foreign Blood Arab horses. https://jvlr.semnan.ac.ir/article_10537_5dc2596e6bd8d9a511a4af7e6d1fef81.pdf